Expression changes of serum HMGB-1 and SOX-4 levels in patients with unexplained recurrent abortion and their clinical significance
Objective To investigate the changes of serum high-mobility group protein B1(HMGB-1)and SRY-re-lated high-mobility-group box 4(SOX4)levels in patients with unexplained recurrent abortion(URSA),and their clinical significance.Methods Ninety-eight URSA patients(URSA group)and 62 healthy pregnant women(control group)were selected.Serum HMGB-1 level was detected by enzyme-linked immunosorbent assay(ELISA)and SOX-4 expression was detected by real-time fluorescence quantitative PCR,and the proportions of peripheral blood helper T cells(Th)17 and regulatory T cells(Treg)were detected.Pearson was used to analyze the correlation between serum HMGB-1 and SOX-4 expression and Th17 proportion,Treg proportion and Th17/Treg ratio in URSA patients.The recurrent pregnancy loss of URSA patients was analyzed,and the factors affecting the recurrent pregnancy loss of URSA patients were ana-lyzed,and the value of HMGB-1 and SOX-4 in predicting the recurrent pregnancy loss of URSA patients was analyzed.Results Serum HMGB-1 level,the proportion of Th17,and Th17/Treg ratio in peripheral blood of the URSA group were higher than those of the control group(all P＜0.05),and the SOX-4 mRNA expression and the proportion of Treg in periph-eral blood were lower than those in the control group(all P＜0.05).Serum HMGB-1 level in URSA patients was positively correlated with the proportion of Th17 and Th17/Treg ratio in the peripheral blood(r=0.512,0.628;both P＜0.05),and was negatively correlated with the proportion of Treg(r=-0.365,P＜0.05).The expression of SOX-4 was negatively cor-related with the proportion of Th17 and Th17/Treg ratio in peripheral blood(r=-0.468,-0.519,respectively;both P＜0.05),and was positively correlated with the proportion of Treg(r=0.502,P＜0.05).High Th17/Treg ratio,high HMGB-1 and more than three miscarriages were risk factors for recurrent pregnancy loss in URSA patients(all P＜0.05),and high SOX-4 was a protective factor(P＜0.05).The area under the curve of HMGB-1 and SOX-4 in predicting URSA adverse pregnancy outcome was 0.787 and 0.757,respectively,and the area under the curve of them combined in predic-tion was 0.912,which was higher than that of either alone(both P＜0.05).Conclusion Serum HMGB-1 level increas-es and SOX-4 expression decreases in URSA patients,which is related to Th17/Treg imbalance and recurrent pregnancy loss.The combined detection of HMGB-1 and SOX-4 is of high value in predicting recurrent pregnancy loss in patients with URSA.
unexplained recurrent spontaneous abortionimmune imbalancehigh-mobility group box-1SRY-re-lated high-mobility-group box 4
万方数据
维普
