Relationships between peripheral blood Th1/Th2 cytokines and cerebral function impairment and cognitive impairment in patients with acute ischemic stroke
Objective To investigate the relationships between peripheral blood T helper cell(Th)1/Th2 cytokines and cerebral function impairment and post-stroke cognitive impairment(PSCI)in patients with acute ischemic stroke(AIS).Methods Totally 125 patients with AIS(AIS group)and 65 healthy individuals in the same period(control group)were selected.The patients with AIS were divided into the mild cerebral function impairment group(33 cases),moderate cerebral function impairment group(74 cases),and severe cerebral function impairment group(18 cases)ac-cording to the score of national institutes of health stroke scale(NIHSS).According to whether PSCI occurred after 6 months,they were assigned into the PSCI group(52 cases)and non-PSCI group(73 cases).Flow cytometry was used to detect the ratio of Th1 and Th2 in peripheral blood,and ELISA was used to detect serum interferon-γ(IFN-γ)and inter-leukin(IL)-4 levels.The correlation between peripheral blood Th1/Th2 cytokines and NIHSS scores in patients with AIS was analyzed by Spearman's correlation,and the factors influencing PSCI in patients with AIS were analyzed by univariate and multivariate Logistic regression.Results Peripheral blood Th1,IFN-γ,and Th1/Th2 were higher in the AIS group than in the control group,and peripheral blood Th2 and IL-4 levels were lower than those in the control group(all P＜0.05).Peripheral blood Th1,IFN-γ,and Th1/Th2 sequentially increased in the mild cerebral functional impairment group,moderate cerebral functional impairment group,and severe cerebral functional impairment group,and peripheral blood Th2 and IL-4 levels sequentially decreased(all P＜0.05).Peripheral blood Th1,IFN-γ,and Th1/Th2 were posi-tively correlated with NIHSS scores in AIS patients,and Th2 and IL-4 were negatively correlated with NIHSS scores(all P＜0.05).The PSCI group was older than the non-PSCI group,with higher NIHSS scores and Th1,IFN-γ,and Th1/Th2,and lower Th2 and IL-4 levels(all P＜0.05).Increased NIHSS score,elevated Th1,elevated IFN-γ,and elevated Th1/Th2 were independent risk factors for PSCI in patients with AIS,and elevated Th2 and IL-4 were independent protective factors(all P＜0.05).Conclusion Peripheral blood Th1/Th2 cytokines are closely associated with the exacerbation of brain functional impairment and the occurrence of PSCI in patients with AIS.
万方数据
维普
