Correlations between serum miR-149-5p and Notch1 mRNA expression and Treg/Th17 balance in patients with allergic rhinitis
Objective To investigate the correlations between the expression levels of miR-149-5p and Notch1 mRNA in serum and the balance of regulatory T cells(Treg)/helper T cells(Th)17 in patients with allergic rhinitis(AR).Methods Ninety-eight AR patients(AR group)and 30 healthy volunteers(control group)were selected.According to the severity of the disease,the patients with AR were divided into the mild group(51 cases)and moderate and severe group(47 cases).Real-time fluorescence quantitative PCR was used to detect the expression of miR-149-5p and Notch1 mRNA in serum,and flow cytometry was used to detect Treg,Th17 and their cytokines in peripheral blood.Pearson correlation was used to analyze the correlations between serum miR-149-5p,Notch1 mRNA expression and peripheral blood Treg,Th17 and their cytokines,as well as the relationship between the miR-149-5p and Notch1 mRNA expression.Results Serum miR-149-5p expression,interleukin(IL)-10,transforming growth factor-β1(TGF-β1)level,peripheral blood Treg cells and Treg/Th17 ratio in the AR group were lower than those in the control group(all P＜0.05),and serum Notch1 mRNA ex-pression,IL-6,IL-17,IL-22,IL-23 and Th17 cells in peripheral blood were higher than those in the control group(all P＜0.05).The expression of miR-149-5p,the levels of IL-10,TGF-β1,Treg cells and Treg/Th17 ratio in peripheral blood were lower in the moderate and severe group than in the mild group(all P＜0.05),and the expression of Notch1 mRNA,the levels of IL-6,IL-17,IL-22,IL-23 and Th17 cells in peripheral blood were higher than those in the mild group(all P＜0.05).Serum miR-149-5p expression in the AR group was positively correlated with peripheral blood Treg cells,Treg/Th17 ratio,serum IL-10,and TGF-β1 levels(r=0.367,0.539,0.265,0.301,respectively;all P＜0.05),and was nega-tively correlated with the levels of serum IL-6,IL-17,IL-22,IL-23 and peripheral blood Th17 cells(r=-0.289,-0.213,-0.336,-0.243,-0.221,respectively,all P＜0.05).Notch1 mRNA expression was negatively correlated with peripheral blood Treg cells,Treg/Th17 ratio,serum IL-10,and TGF-β1 level(r=-0.346,-0.508,-0.281,-0.293,respectively;all P＜0.05),and was positively correlated with serum IL-6,IL-17,IL-22,IL-23 levels and peripheral blood Th17 cells(r=0.326,0.241,0.302,0.285,0.237,respectively,all P＜0.05).Serum miR-149-5p was negatively correlated with Notch1 mRNA expression(r=-0.550,P＜0.05).Conclusion The expression of miR-149-5p is down-regulated and Notch1 mRNA is up-regulated in AR patients,which are related to the imbalance of Treg/Th17 in AR patients.
allergic rhinitismicroRNAmiR-149-5pNotch1 signaling pathwayregulatory T cellshelper T cell 17
万方数据
维普
