Therapeutic effect and mechanism of intragastric administration of Yiqi Liyan formula on mice with radioactive salivary gland injury
Objective To investigate the therapeutic effect and mechanism of Yiqi Liyan formula on mice with radio-active salivary gland injury.Methods Sixty healthy male C57BL/6J mice were divided into the blank group,model group,low-dose Yiqi Liyan formula group(100 mg/kg)and high-dose Yiqi Liyan formula group(200 mg/kg),with 15 mice in each.Except for the blank group,all mice were irradiated with 15 Gy through an electron linear gas pedal to es-tablish the radioactive salivary gland injury models,and were given the corresponding drugs by gavage for 2 weeks after successful treatment.At the end of the experiment,secreted saliva was collected;HE staining was used to detect the pathological structure of submandibular gland;transmission electron microscopy was used to examine the ultrastructure of submandibular gland and the formation of autophagosomes;and Western blotting was used to measure the expression lev-els of c-Jun N-terminal kinase(JNK),Beclin 1,LC3-Ⅰ,LC3-Ⅱ,and p62 in the submandibular gland tissues.Results Compared with the blank group,the weight and salivary secretion of the submandibular gland of mice in the model group were reduced;the area of the glandular vesicles increased,the number of granular convoluted tubules de-creased,and fibrosis appeared around the ducts;the ultrastructure showed nuclear pyrolysis,formation of autophagic ves-icles,disruption of the endoplasmic reticulum,and swelling of the mitochondria;and the protein expression levels of p-JNK/JNK,Beclin 1,and LC3Ⅱ/Ⅰ increased,and the expression of p62 protein decreased(all P＜0.05).Compared with the model group,the weight of submandibular gland and salivary secretion of mice in the low-dose and high-dose Yiqi Liyan formula groups gradually increased with the concentrations of the drug administered;the pathology and ultra-structure improved,and the formation of autophagic vesicles was reduced;and the protein expression levels of p-JNK/JNK,Beclin 1,and LC3Ⅱ/Ⅰ gradually decreased with the concentrations of the drug,and the protein expression of p62 gradually increased(all P＜0.05).Conclusion Yiqi Liyan formula may inhibit autophagy by activating JNK/Beclin 1 pathway to achieve ameliorative effects on radioactive salivary gland injury in mice.
万方数据
维普
