Effect and mechanism of miR-223 on proliferation,cell cycle,apoptosis,and inflammatory response of psoriasis cells
Objective To investigate the effects of expression of microRNA-223(miR-223)on cell proliferation,cell cycle,apoptosis and inflammatory reaction of psoriasis and their mechanism.Methods Human immortalized kerati-nocytes HaCaT were cultured in vitro.The cells were divided into the control group,model group,model+miR-NC group,model+miR-223 inhibitor group.Except for the control group,cells in the other groups were induced to establish psoriasis cell models by M5 method.Real-time quantitative PCR(RT-Qpcr)was used to detect the expression of miR-223 in each group.CCK-8 method and flow cytometry were used to detect cell proliferation activity,cell cycle,and apoptosis in each group.ELISA was used to detect the levels of inflammatory factors such as IL-6,IL-1β and TGF-β1 in supernatant.West-ern blotting was used to detect the expression of phosphatase and tensin homolog deleted on chromosome ten(PTEN)pro-tein.Results Compared with the control group,the expression level of miR-223 significantly increased(P＜0.05),OD values at 48,72 and 96 h and the proportion of cells in the cell cycle S phase significantly increased(all P＜0.05),the proportion of cells in the cell cycle G0/G1 and apoptosis rate significantly decreased(all P＜0.05),the levels of IL-6,IL-1β and TGF-β1 significantly increased(all P＜0.05),while the expression of PTEN protein decreased in the model group(P＜0.05).Compared with the model+miR-NC group,the expression level of miR-223 inhibitor group significant-ly decreased(P＜0.05),OD values at 48,72 and 96 h and the proportion of cells in the S phase decreased(P＜0.05),the proportion of cells in the cell cycle G0/G1 and apoptosis rate significantly increased(P＜0.05),the levels of IL-6,IL-1β and TGF-β1 significantly decreased(P＜0.05),and PTEN protein expression increased in the model+miR-223 inhibitor group(P＜0.05).There were no significant differences in the above indexes between the model group and the model+miR-NC group(all P＞0.05).Conclusions The expression level of miR-223 increases in psoriasis cells.Down-regulating the level of miR-223 can inhibit the proliferation and inflammatory response of psoriasis cells,and promote their apoptosis and block the cell cycle in G0/G1 phase,which may be related to its regulation of PTEN.
psoriasismicroRNA-223HaCaT cellscell proliferationcell cycleapoptosisinflammatory re-actionsphosphatase and tensin homolog deleted on chromosome ten
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