Expression changes of serum exosomes lncRNA-HOXA-AS3 and miR-29a in prostate cancer patients and their relationships with clinical characteristics and prognosis of patients
Objective To observe the expression changes of serum exosome long non-coding RNA HOXA-AS3(lncRNA HOXA-AS3)and microRNA 29a(miR-29a)in patients with prostate cancer(PCa),and to analyze their rela-tionships with clinical characteristics and prognosis of patients.Methods Totally 115 PCa patients(observation group)and 115 healthy individuals(control group)undergoing physical examinations at the same time were enrolled.The expres-sion levels of lncRNA-HOXA-AS3 and miR-29a in serum exosomes were detected by qRT-PCR.The correlation between the two indicators and their relationships with the clinical features of patients were analyzed by Pearson correlation analy-sis.The observation group was divided into the high lncRNA-HOXA-AS3 expression group(56 cases)and low lncRNA-HOXA-AS3 expression group(59 cases)according to the mean relative expression of lncRNA-HOXA-AS3,and divided into the high miR-29a expression group(58 cases)and low miR-29a expression group(57 cases)according to the mean relative expression of miR-29a.The survival curves were drawn by Kaplan-Meier method,and the survival rates of high expression group and low expression group were compared by Log-rank test.The risk factors for poor prognosis of PCa pa-tients were analyzed by Cox regression analysis.Results Compared with the control group,the relative expression level of serum exosome lncRNA-HOXA-AS3 in the observation group increased,while the relative expression level of miR-29a decreased(both P<0.05).The expression of lncRNA-HOXA-AS3 and miR-29a in the serum exosomes of the observation group was negatively correlated(r=-0.689,P<0.05).The expression of lncRNA-HOXA-AS3 and miR-29a in serum exosomes was related to Gleason score,T stage,and lymph node metastasis in PCa patients(all P<0.05).Compared with patients with low expression of lncRNA-HOXA-AS3,patients with high expression of lncRNA-HOXA-AS3 had a low-er survival rate(P<0.05);compared with those with high expression of miR-29a,patients with low expression of miR-29a had a lower survival rate(P<0.05).Univariate and multivariate analysis showed that lncRNA-HOXA-AS3 and miR-29a were independent risk factors for poor prognosis in patients with PCa(P<0.05).Conclusions The expression of ln-cRNA-HOXA-AS3 in the serum exosomes of PCa patients increases,while the expression of miR-29a is down-regulated,and the two expression levels are negatively correlated.Both indicators are related to the patient's Gleason score,T stage,and lymph node metastasis.Patients with high expression of lncRNA-HOXA-AS3 and low expression of miR-29a have a low survival rate.The lncRNA-HOXA-AS3 and miR-29a are independent risk factors for poor prognosis in PCa patients.
万方数据
维普
