Effects of silencing miR-126 on proliferation,apoptosis,and inflammatory response of osteoarticular chondrocytes
Objective To investigate the effects of silencing miR-126 on proliferation,apoptosis,and inflammatory response of osteoarticular chondrocytes and their mechanism.Methods Human osteoarticular chondrocytes HC-a were cultured in vitro.HC-a cells in the third generation,which were in the logarithmic growth phase and had good growth sta-tus,were randomly divided into the control group,IL-1β group,IL-1β+miR-NC group,and IL-1β+miR-126 inhibitor group,respectively.Cells in the IL-1β+miR-NC group and IL-1β+miR-126 inhibitor group were transfected with miR-NC and miR-126 inhibitor,respectively.Except for the control group,the other three groups were treated with 10 ng/mL IL-1β induction to construct the cell models of osteoarthritis(OA).The cells of each group were collected,and the expres-sion of miR-126 was detected by RT-qPCR,cell proliferation activity was detected by CCK-8,apoptosis rate was detected by flow cytometry,and inflammatory factors TNF-α,IL-6 and IFN-γ in the culture supernatant were detected by ELISA.The expression levels of SIRT1 and Caspase-3 proteins were detected by Western blotting.Results Compared with the control group,the relative expression of miR-126 increased,the cell proliferation activity decreased,apoptosis rate increased,the levels of TNF-α,IL-6 and IFN-γ in culture supernatance increased,the relative expression of SIRT1 pro-tein decreased,and the relative expression of Caspase-3 protein increased in the IL-1β group(all P<0.05).Compared with the IL-1β+miR-NC group,the relative expression decreased,cell proliferation activity increased,apoptosis rate decreased,the levels of TNF-α,IL-6 and IFN-γ in culture supernatant decreased,the relative expression of SIRT1 pro-tein increased,and the relative expression of Caspase-3 protein decreased in the miR-126 inhibitor group(all P<0.05).There were no significant differences in the above indexes between IL-1β group and IL-1β+miR-NC group(all P>0.05).Conclusion Silencing miR-126 can promote the proliferation of OA cells and inhibit apoptosis and inflammation,which may be related to the regulation of SIRT1 protein expression.
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