Radiosensitizing effect of miR-448 on lung adenocarcinoma A549 cells and its mechanism
Objective To investigate the radiosensitizing effect of miR-448 on lung adenocarcinoma A549 cells and to explore its mechanism of action.Methods Lung adenocarcinoma A549 cells were randomly divided into the blank control group,radiotherapy group,radiotherapy+miR-448 mimics group,and radiotherapy+NC mimics group,respective-ly.Cells in the radiotherapy+miR-448 mimics group and the radiotherapy+NC mimics group were first transfected with miR-448 mimics and NC mimics for 24 h,respectively,and then were irradiated with radiotherapy.Cells in the radiothera-py group were only irradiated with radiotherapy,while cells in the blank control group were cultured normally.MTT assay and cell cloning assay were used to detect the proliferation activity and cell survival of cells in the four groups,Transwell assay was used to detect cell migration and invasion ability,flow cytometry was used to detect the apoptosis,Western blot-ting was used to detect TBX3 and PTEN proteins,qPCR was used to detect miR-448 and TBX3,phosphatase and tensin homolog(PTEN)mRNA,and dual luciferase assay was used to identify the binding sites between PTEN and miR-448.Results Compared with the blank control group,the cell proliferation,migration and invasion abilities decreased,and the apoptosis increased in the radiotherapy group(all P<0.05).Compared with the radiotherapy group and the radiothera-py+NC mimics group,the cell proliferation,migration and invasion abilities decreased,and the apoptosis increased in the radiotherapy+miR-448 mimics group(all P<0.05).Compared with the radiotherapy group and the radiotherapy+NC mimics group,the expression level of PTEN protein increased in the radiotherapy+miR-448 mimics group(both P<0.05).Compared with the radiotherapy group and the radiotherapy+NC mimics group,the PTEN protein and mRNA levels in-creased and the TBX3 mRNA level decreased in the radiotherapy+miR-448 mimics group(all P<0.05).MiR-448 specifi-cally bound to the 3'UTR of PTEN.Conclusion The miR-448 can enhance the radiation sensitivity of lung cancer A549 cells probably by regulating the expression levels of TBX3 and PTEN;phosphatase and tensin homolog.
lung neoplasmslung adenocarcinoma cellsmicroRNAradiotherapyTBX3phosphatase and tensin homolog
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