Expression changes of Notch1 signaling pathway-related proteins in brain tissues of immature SD rats with acute hypoxic-ischemic brain injury and their effect on neuronal apoptosis
Objective To observe the expression changes of Notch1 signaling pathway-related proteins(transcrip-tion factor notch1,NICD,and its downstream protein HES1),and the apoptosis of neurons after inhibition of Notch1 sig-naling pathway in brain tissues of immature SD rats with acute hypoxic-ischemic brain injury,so as to investigate the role and mechanism of Notch1 signaling pathway in neonatal brain injury.Methods Eighteen three-day-old Sprague-Dawley rats were randomly divided into the Notch1 inhibition group,model group,and control group(6 rats),respectively.Rats in the Notch1 inhibition group were intraperitoneally injected with DAPT(100 mg/kg)30 min before modeling,and then were treated with ischemia and hypoxia.Rats in the model group were intraperitoneally injected with 1%DMSO before hy-poxia and ischemia treatment.Rats in the control group received sham surgery.Notch1 signaling pathway proteins(notch1,NICD,and HES1)and apoptotic proteins(Caspas-3,Bax,and Bcl-2)were detected by Western blotting.The changes of brain structure and apoptosis were observed by Niss staining and TUNEL staining.Results Compared with the control group,the expression levels of transcription factor NICD of Notch1 signaling pathway and its downstream regula-tory protein HES1 increased(both P<0.05),the expression level of pro-apoptotic protein Caspas-3 increased(P<0.05),and the expression level of anti-apoptotic protein Bcl-2 decreased in the model group(P<0.05).Compared with the model group,the expression levels of NICD and HES1 protein decreased(both P<0.05),the expression level of Caspas-3 pro-tein decreased(P<0.05),and the expression level of Bcl-2 increased in the Notch1 inhibition group(P<0.05).Com-pared with the model group,the brain tissue structure of the Notch1 inhibition group was significantly improved and the number of apoptotic neurons decreased(both P<0.05).Conclusions Notch1 signaling pathway-related proteins NICD and HES1 increased,the expression of pro-apoptotic protein Caspase-3 increased,and the expression of anti-apoptotic pro-tein Bcl-2 decreased in SD rats with acute hypoxic-ischemic brain injury.After the inhibition of Notch1 signaling pathway,the apoptosis of nerve cells decreased in SD rats with acute hypoxic-ischemic brain injury.Notch1 signaling pathway in-duced the neonatal brain injury by promoting nerve cell apoptosis in the brain tissues.
国家科技期刊平台
NSTL
万方数据
