Proliferation,apoptosis,invasion,and migration of fibroblasts in human conjunctiva and thoracic skin pathological scars cultured in resveratrol-containing medium
Objective To observe the proliferation,apoptosis,invasion,and migration of human ocular conjuncti-val pterygium(ocular pathological scar)fibroblasts and human anterior thoracic skin pathological scars(skin-derived path-ological scars)fibroblasts cultured in resveratrol-containing medium,in order to investigate the effects of resveratrol on the proliferation,apoptosis,invasion,and migration of human ocular pathological scar fibroblasts and skin-derived pathologi-cal scar fibroblasts.Methods Primary human ocular pathological scar fibroblasts and skin-derived pathological scar fi-broblasts were isolated by tissue block adherent method.The cell viability(OD value)of human ocular and skin-derived pathological scar fibroblasts was detected by CCK-8 when the cells were cultured in media containing 0(control),1,2,4,8,16,32,64,128 and 256 µg/mL resveratrol for 48 h.Human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts were cultured with 0 and 75 µg/mL resveratrol for 8 h,and the apoptosis rate was measured by Tunel method.These two kinds fibroblasts were cultured in 0 and 30 µg/mL resveratrol media for 48 h,and the migra-tion and invasion abilities of the cells(the number of invasive migratory cells)were observed.Results The OD values of human ocular pathological scar fibroblasts cultured in media containing 128 and 256 µg/mL resveratrol decreased(both P<0.05),and the OD values of skin-derived pathological scar fibroblasts cultured in media containing 64,128,and 256 µg/mL resveratrol all decreased(all P<0.05),in comparison with those at 0 µg/mL resveratrol.Compared with those at 0 µg/mL resveratrol,the apoptosis rates of human ocular pathological scar fibroblasts and skin-derived pathological scar fi-broblasts cultured in 75 µg/mL resveratrol medium increased(all P<0.05),and the number of invasion and migration cells of human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts cultured in medium con-taining 30 µg/mL resveratrol became smaller(all P<0.01).Conclusions Human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts cultured in resveratrol-containing medium have reduced viability,inva-sion,and migration abilities,and increased apoptosis rate.Resveratrol can significantly inhibit the proliferation,inva-sion,and migration and promote the apoptosis of human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts.
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