Diagnostic value of serum PDCD4 and HSP70 levels in lymph node metastasis of early cervical cancer
Objective To investigate the diagnostic value of serum programmed cell death protein 4(PDCD4)and heat shock protein 70(HSP70)levels in lymph node metastasis(LNM)of early cervical cancer,in order to provide more evidence for the diagnosis of LNM of early cervical cancer.Methods A total of 132 patients with early cervical cancer(observation group)were selected,and sentinel lymph node biopsy was performed during the operation.Histopathological examination was performed to determine whether LNM occurred,and 60 healthy female volunteers(control group)were selected during the same period.Fasting peripheral venous blood of all subjects was collected and serum was retained by cen-trifugation.Serum PDCD4 and HSP70 levels were detected by ELISA.The clinical data of patients with early cervical cancer were collected and the risk factors for LNM of early cervical cancer were analyzed by multivariate Logistic regression model.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of serum PDCD4 and HSP70 levels in LNM of early cervical cancer.Results The serum PDCD4 level in the observation group was lower than that in the control group,and the serum HSP70 level was higher than that in the control group(both P<0.05).Among 132 patients with early cer-vical cancer,25 had LNM and 107 did not have LNM.Univariate analysis showed that maximum tumor diameter,depth of cer-vical interstitial infiltration,FIGO stage and serum PDCD4 and HSP70 levels might be related to LNM of early cervical can-cer(all P<0.05).Multivariate Logistic regression analysis showed that cervical interstitial infiltration depth≥1/2 cervical muscle wall,FIGO stage IIA and increased serum HSP70 level were independent risk factors for LNM of early cervical can-cer,while increased serum PDCD4 level was independent protective factor(all P<0.05).ROC curve analysis showed that the area under the curve(AUC)of serum PDCD4 and HSP70 levels alone and combined in predicting LNM of early cervical cancer were 0.810,0.817 and 0.876,respectively,and the AUC of serum PDCD4 and HSP70 levels combined in predict-ing LNM of early cervical cancer was greater than that of the two alone(both P<0.05).Conclusions The decrease of serum PDCD4 level and the increase of serum HSP70 level are closely related to LNM of early cervical cancer.Serum PDCD4 and HSP70 levels have certain diagnostic value for early cervical cancer LNM,and their combined diagnosis value is higher.
early cervical cancerprogrammed cell death protein 4heat shock protein 70lymph node metasta-sisdiagnostic value
万方数据
维普
