敲减辅酶Q10B表达对裸鼠食管鳞癌皮下移植瘤细胞增殖、凋亡及上皮间质转化的影响

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中文摘要：目的探讨敲减辅酶Q10B(COQ10B)表达对裸鼠食管鳞状细胞癌(鳞癌)皮下移植瘤细胞增殖、凋亡及上皮间质转化(EMT)的影响。方法利用慢病毒转染技术将COQ10B的干扰慢病毒及其阴性对照转染至食管鳞癌细胞(KYSE150细胞)中，构建敲减COQ10B表达的KYSE150细胞(sh-COQ10B组)和阴性对照KYSE150细胞(sh-NC组)。选择雌性BALB/c裸鼠20只，随机分入sh-COQ10B组和sh-NC组各10只，分别于裸鼠右前肩胛处皮下接种转染sh-COQ10B和sh-NC的KYSE150细胞，以此构建裸鼠皮下移植瘤模型，观察接种后不同时间皮下移植瘤生长情况并测量瘤体的体积和质量。接种第26天实验结束后取瘤体组织，采用HE、免疫组化、TUNEL染色法观察裸鼠皮下移植瘤中细胞增殖和凋亡的情况，以Western blotting法检测移植瘤中EMT相关蛋白(E-cadherin和Vimentin)和凋亡相关蛋白(Bcl-2和Bax)表达。结果与sh-NC组比较，sh-COQ10B组中COQ10B蛋白表达量低(P<0。05);皮下移植瘤模型构建成功且裸鼠均无死亡。sh-COQ10B组移植后不同时间瘤体体积和质量均小于sh-NC组(P均<0。05)。与sh-NC组比较，sh-COQ10B组肿瘤细胞凋亡率高，瘤体组织内Bcl-2、Vimentin蛋白表达低，Bax、E-cadherin表达高，差异均有统计学意义(P均<0。05)。结论敲减COQ10B表达可抑制裸鼠食管鳞癌皮下移植瘤细胞增殖和EMT的发生，促进肿瘤细胞凋亡。

外文标题：Effects of knocking down coenzyme Q10B on proliferation,apoptosis and EMT of esophageal squamous cell carcinoma xenografts in nude mice

外文摘要：Objective To investigate the effects of coenzyme Q10B(COQ10B)knockdown on the proliferation,apoptosis and epithelial-mesenchymal transition(EMT)of esophageal squamous cell carcinoma xenografts in nude mice.Methods The interference lentivirus of COQ10B and its negative control were transfected into KYSE150 cells by lentivi-rus transfection technology.The KYSE150 cells with knockdown of COQ10B expression(sh-COQ10B group)and the nega-tive control KYSE150 cells(sh-NC group)were constructed.Twenty female BALB/c nude mice were randomly divided in-to the sh-COQ10B group(n=10)and sh-NC group(n=10).KYSE150 cells transfected with sh-COQ10B and sh-NC were subcutaneously injected into the right anterior scapula of nude mice to establish subcutaneous transplantation tumor mod-els.The growth of subcutaneous xenografts at different time after inoculation was observed and the size and the weight of tu-mors were measured.At the end of the experiment on the 26th day after inoculation,tumor tissues were taken,HE,immu-nohistochemistry,and TUNEL staining methods were used to observe cell proliferation and apoptosis in subcutaneous xeno-grafts in nude mice.Western blotting was used to detect the expression levels of EMT-related proteins(E-cadherin and Vi-mentin)and apoptosis-related proteins(Bcl-2 and Bax).Results Compared with the sh-NC group,the protein expres-sion of COQ10B in the sh-COQ10B group decreased(P<0.05).The subcutaneous transplanted tumor models were estab-lished successfully and no death occurred in nude mice.The tumor volume and mass in the sh-COQ10B group were lower than those in the sh-NC group(both P<0.05).Compared with the sh-NC group,the apoptosis rate of tumor cells in the sh-COQ10B group increased,the protein expression levels of Bcl-2 and Vimentin in tumor tissues decreased,and the expres-sion levels of Bax and E-cadherin increased,with statistically significant difference(all P<0.05).Conclusion Knock-ing down COQ10B expression can inhibit the proliferation of subcutaneous transplanted tumor and the occurrence of EMT in nude mice,and promote the apoptosis of tumor cells.

外文关键词：

esophageal squamous cell carcinomaxenografts in nude micecoenzyme Q10Bcell proliferationapoptosisepithelial-mesenchymal transition

作者：

魏瑜、李志芳、曹雷雨、高艳、马晓丽、张莉

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作者单位：

新疆医科大学第一附属医院综合内四科/特需内科,乌鲁木齐 830054

关键词：

食管鳞状细胞癌裸鼠皮下移植瘤辅酶Q10B 细胞增殖细胞凋亡上皮间质转化

基金：

省部共建中亚高发病成因与防治国家重点实验室开放课题资助项目新疆维吾尔自治区重点研发计划项目新疆维吾尔自治区重点研发计划项目新疆维吾尔自治区自然科学基金资助项目

项目编号：

SKL-HIDCA-2022-SG12020B030032020B03003-32022D01C783

出版年：

2024

DOI：

10.3969/j.issn.1002-266X.2024.28.009

山东医药

山东卫生报刊社

山东医药

CSTPCD

影响因子：1.225

ISSN：1002-266X

年,卷(期)：2024.64(28)