Regulatory effects of lncRNA JPX on proliferation and invasion of nasopharyngeal carcinoma cells and the mechanism
Objective To observe the regulatory effects of long non-coding RNA(lncRNA)JPX on the proliferation and invasion of nasopharyngeal carcinoma cells and to explore their mechanism.Methods The nasopharyngeal carcino-ma cell lines CNE-1,5-8F,6-10B,HONE-1,C666-1 and nasopharyngeal normal epithelial cell line NP69 were selected,and we used RT-qPCR to detect the expression of lncRNA JPX in the cells.C666-1 cells with the highest expression of ln-cRNA JPX were taken as the study cells.C666-1 cells were randomly divided into the JPX down-expression group,down-expression control group,high JPX expression group,and high expression control group,respectively.They were trans-fected with JPX silencing sequence sh JPX,negative control sequence sh NC,JPX over-expression plasmid pcDNA3.1-JPX,and negative control plasmid pcDNA 3.1-NC,respectively.MTT assay was used to observe cell proliferation ability,and Transwell assay was used to observe cell invasion ability.We used the StarBase database to predict the targeted miR-NAs and genes of lncRNA JPX,and found that lncRNA JPX had a targeted binding site with miR-1265,and miR-1265 had a targeted binding site with MAT1;the dual luciferase assay was used to verify the targeted relationship,and it showed that lncRNA JPX could bind to miR-1265 and inhibit miR-1265 expression,and miR-1265 could bind to MAT1 and inhib-it MAT1 expression.Further,C666-1 cells were randomly divided into the control group,JPX down-regulation group,JPX down-regulation+miR-1265 down-regulation group,and JPX down-regulation+MAT1 down-regulation group,and were transfected with NC control sequence,sh JPX sequence,sh JPX+miR-1265 inhibitor sequence,and sh JPX+si-MAT1 sequence,respectively.Western blotting was used to detect the expression of MAT1 protein in cells of each group,MTT assay was used to observe cell proliferation ability,and Transwell assay was used to observe cell invasion ability.Results Cell viability and the number of invasive cells were as follows:the high JPX expression group>the high expres-sion control group,and down-expression control group>JPX down-expression group(all P<0.05).The MAT1 protein ex-pression,cell viability,the number of invasive cells were as follows:control group,JPX down-regulation+miR-1265 down-regulation group>JPX down-regulation group>JPX down-regulation+MAT1 down-regulation group(all P<0.05).Conclusion LncRNA JPX can promote the proliferation and invasion of nasopharyngeal carcinoma cells,and its mecha-nism may be related to targeted regulation of the miR-1265/MAT1 axis.
long non-coding RNA JPXnasopharyngeal carcinomacell proliferationcell invasionmicroRNA-1265MAT1
万方数据
维普
