Ameliorative effect and mechanism of apigenin on lipopolysaccharide-induced injury of mouse hippocampal neuronal cells
Objective To observe the ameliorative effect of apigenin on lipopolysaccharide(LPS)-induced injury of hippocampal neuron cells in mice,and to explore its related mechanism.Methods Mouse hippocampal neuron cell line HT22 was cultured in vitro,and the optimal concentrations of LPS and apigenin were screened by CCK-8 method.The opti-mal concentrations of LPS and apigenin were 2 μg/mL and 0.25 μmol/L,respectively.HT22 cells were divided into the control group,model group and apigenin group.Cells in the control group were cultured normally without any treatment,cells in the model group were cultured by adding 0.25 μmol/L LPS,and cells in the apigenin group were cultured by inter-vening with 2 μg/mL of LPS for 24 h and then adding 0.25 μmol/L apigenin.The apoptosis was observed by Annexin V/PI double staining,and the cellular tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)proteins were detected by Western blotting.UPLC-Q-TOF-MS/MS was used to analyse and screen the differential metabolites of the cells in each group,and the differential metabolites were subjected to MetPA analysis to obtain their potential metabolic pathways.Re-sults The apoptosis rates and the expression levels of TNF-α and IL-1β proteins were as follows:the model group>the apigenin group>the control group(all P<0.01).Metabolomic analysis screened out 16 different metabolites,such as uri-dine diphosphate glucose,glutamic acid,succinic acid,citric acid and L-carnitine,which involved 28 metabolic path-ways such as linolic acid,alanine,aspartic acid and glutamic acid metabolism,TCA cycle,and glutathione metabolism.Conclusion Apigenin can ameliorate LPS-induced mouse hippocampal neuronal cell injury,and its mechanism may be related to the regulation of intracellular metabolic disorders.
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