Immediate analgesic effect and mechanism of Three-Manipulation and Three-Acupoint tuina on rats with sciatic nerve injury
Objective To observe the immediate analgesic effect and mechanism of Three-Manipulation and Three-Acupoint tuina on rats with sciatic nerve injury.Methods Twenty-seven Sprague-Dawley(SD)rats were randomly divided into the sham operation group,model group,and tuina group,with nine rats in each group.In the model and tuina groups,we established the models of mild chronic constriction injury of sciatic nerve to simulate clinical neuropathic pain,while in the sham operation group,rats only underwent nerve exposure and suturing.On the 7th day after modeling,rats in the tuina group received Three-Manipulation and Three-Acupoint tuina therapy,with each acupoint treated for 1 minute using three techniques(9 minutes in total).Rats in the sham operation and model groups were only restrained without intervention for 9 minutes.Mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)were measured before modeling(T1),after modeling but before tuina(T2),and after tuina(T3)to evaluate the immediate analgesic effect.After evalua-tion,lumbar enlargement segment spinal dorsal horn tissues were collected.Western blotting was used to detect the expres-sion of brain-derived neurotrophic factor(BDNF),tyrosine kinase receptor B(TrkB),and potassium-chloride cotransporter 2(KCC2)proteins,and immunofluorescence was used to detect the ionized calcium-binding adapter molecule 1(IBA-1),cluster of differentiation 68(CD68),and cellular oncogene Fos(c-Fos)expression.Results Compared with the sham op-eration group,the model group showed significantly lower MWT and TWL at both T2 and T3(both P<0.05).However,the tuina group had significantly higher MWT and TWL at T3 as compared with the model group(both P<0.05).The expression levels of BDNF and TrkB in the dorsal horn were higher,and KCC2 expression was lower in the model group than in the sham operation group(all P<0.05).Compared with the model group,the tuina group exhibited lower BDNF and TrkB expression but higher KCC2 expression(all P<0.05).No significant differences were found in the expression of BDNF,TrkB,or KCC2 between the sham operation and tuina groups(all P>0.05).Additionally,the fluorescence intensity of IBA-1,CD68,and c-Fos was higher in the model group than in the sham operation group(all P<0.05),while CD68 and c-Fos expression was lower in the Tuina group as compared with the model group(both P>0.05).Conclusions Three-Manipu-lation and Three-Acupoint tuina demonstrates a good immediate analgesic effect in rats with sciatic nerve injury.The under-lying mechanism may involve modulating microglia-neuron interactions and altering the activation state of microglia.
sciatic nerve injuryThree-Manipulation and Three-Acupointtuinaimmediate analgesic effectmicroglianeuronsrats
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