Relationships of expression of miR-143-3p and TIMELESS mRNA in epithelial ovarian cancer tissues with clinicopathological characteristics and prognosis of patients
Objective To investigate the relationships of expression levels of microRNA-143-3p(miR-143-3p)and timeless circadian regulator(TIMELESS)mRNA in epithelial ovarian cancer(EOC)tissues with the clinicopathological characteristics and prognosis of patients.Methods We selected 106 patients with EOC,and the EOC tissues and corre-sponding adjacent tissues were collected.Real-time fluorescence quantitative PCR was performed to detect miR-143-3p and TIMELESS mRNA expression.The binding sites of miR-143-3p and TIMELESS were predicted by online databases such as TargetScan,StarBase,and miRWalk.Pearson correlation was used to analyze the correlation between miR-143-3p and TIMELESS mRNA.We analyzed the relationships between the expression of miR-143-3p and TIMELESS mRNA and the clinicopathological characteristics of EOC patients.EOC patients were followed up for three years,and we calculated the cumulative survival rate.EOC patients were divided into high/low miR-143-3p and TIMELESS mRNA expression groups according to the mean value of their expression,and Kaplan-Meier method was used to draw survival curves for sur-vival analysis.Multivariate Cox regression analysis was performed to analyze the effects of miR-143-3p and TIMELESS on the prognosis of EOC patients.Results Compared with the adjacent tissues,miR-143-3p was low expressed and TIME-LESS mRNA was highly expressed in EOC tissues(both P<0.05).The miR-143-3p and TIMELESS had a binding site at 3'-untranslated region 1453-1460.The miR-143-3p and TIMELESS mRNA expression in EOC tissues showed a negatively correlated(r=-0.748,P<0.05).The miR-143-3p and TIMELESS mRNA expression was related to International Federa-tion of Gynecology and Obstetrics(FIGO)stage,degree of differentiation,lymph node metastasis,and distant metastasis(all P<0.05).The 3-year overall survival rate of 106 EOC patients was 69.81%(74/106).The high miR-143-3p expres-sion patients had a higher 3-year overall survival rate than the low miR-143-3p expression patients,and the 3-year overall survival rate of patients with high TIMELESS mRNA expression was lower than that of patient with low TIMELESS mRNA expression patients(both P<0.05).Independent risk factors for death in EOC patients were FIGO stage Ⅲ-Ⅳ,poor differ-entiation,lymph node metastasis,distant metastasis,and TIMELESS mRNA≥1.11,and the independent protective fac-tor was miR-143-3p≥0.97(all P<0.05).Conclusion In EOC tissues,miR-143-3p was low expressed and TIMELESS mRNA was highly expressed,and their expression changes were closely associated with FIGO stage,degree of differentia-tion,lymph node metastasis,distant metastasis and prognosis.
万方数据
维普
