Significance of NRG-1 gene rs35753505 polymorphism and changes of serum NRG-1 level in children with focal epilepsy
Objective To investigate the significance of neuregulin 1(NRG-1)gene rs35753505 polymorphism and changes of serum NRG-1 level in children with focal epilepsy.Methods Totally 101 children with focal epilepsy(epi-lepsy group)and 101 healthy children(control group)were selected.According to the curative effect,the children with epilepsy were divided into the effective group(n=71)and ineffective group(n=30).The polymorphism of NRG-1 gene rs35753505 was detected by polymerase chain reaction and ligase detection reaction,and NRG-1 was detected by enzyme-linked immunosorbent assay.Multivariate Logistic regression model was used to analyze the effect of serum NRG-1 level on the efficacy of ASM.Results The CC genotype and allele C gene frequencies in the epilepsy group were higher than those in the control group(both P<0.05),and the serum NRG-1 level was lower than that in the control group(P<0.05).The risk of focal epilepsy was higher in CC genotype than in TT(OR=1.658,95%CI:1.506-1.957)and CT(OR=1.404,95%CI:1.205-1.733)genotypes(both P<0.05);the risk of focal epilepsy was higher in C allele than in T al-lele(OR=1.872,95%CI:1.643-2.892,P<0.05).The CC genotype and allele C gene frequencies in the ineffective group were higher than those in the effective group(both P<0.05),and the serum NRG-1 level was lower than that in the effective group(P<0.05).The risk of invalid ASM was higher in CC genotype than in TT(OR=1.358,95%CI:1.106-1.537)and CT(OR=1.274,95%CI:1.075-1.465)genotypes(both P<0.05),and the risk of invalid ASM was higher in C allele than in T allele(OR=1.572,95%CI:1.465-1.432,P<0.05).The serum NRG-1 level in focal epilepsy chil-dren with CC genotype was lower than that of children with CT and TT genotypes(all P<0.05),and there was no signifi-cant difference in serum NRG-1 level between CT and TT genotypes in children with focal epilepsy(P>0.05).The propor-tions of abnormal EEG and family history of epilepsy in the ineffective group were higher than those in the effective group(both P<0.05).Abnormal EEG and CC genotype were risk factors for the efficacy of ASM(P<0.05),and high serum NRG-1 level was a protective factor(P<0.05).Conclusion NRG-1 gene rs35753505 polymorphism and serum NRG-1 level affect the susceptibility of focal epilepsy and the efficacy of ASM.
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