Effects of bone marrow mesenchymal stem cell exosomes on proliferation,migration,BDNF,and VEGF secretion of Schwann cells
Objective To investigate the effects of exosomes secreted by bone marrow mesenchymal stem cells(BM-SCs)on the proliferation,migration,brain-derived neurotrophic factor(BDNF)and vascular endothelial growth factor(VEGF)secretion of Schwann cells.Methods Exosomes secreted by BMSCs were extracted from bilateral femoral bone marrow of 3-week-old SD rats,and Schwann cells were extracted from bilateral sciatic nerves of 3-day-old SD rats.Schwann cells were divided into the control group and BMSCs group.Cells in the control group were cultured with Schwann cell medium,and cells in the BMSCs group were cultured with Schwann cell medium containing 100 μg/mL BMSCs exo-somes.When Schwann cells were cultured for 24,48 and 72 h,the proliferation of Schwann cells in the two groups was ob-served by CCK-8(expressed by OD value).When Schwann cells were cultured for 24 and 48 h,the migration abilities of Schwann cells in the two groups were observed by Scratch test(expressed by migration relative area ratio).When Schwann cells of the two groups were cultured for 72 h,the secretion capacities of BDNF and VEGF were observed by ELISA meth-od(represented by the content of BDNF and VEGF in the cell supernatant).Results The OD values of Schwann cells cultured for 24,48 and 72 h were 1.06±0.04,1.22±0.06 and 1.42±0.08,respectively,in the control group,versus 1.46±0.11,1.70±0.10 and 1.94±0.19,respectively,in the BMSCs group,with statistically significant difference(all P<0.05).The relative area ratios of Schwann cells in the control group were 26.43%±4.75%and 45.44%±3.41%at 24 and 48 h,respectively,and those in the BMSCs group were 46.29%±3.88%and 65.04%±2.85%,respectively,with statistically significant difference(all P<0.05).The content of BDNF in the supernatant of Schwann cells cultured for 72 h in the control group was(72.48±7.25)pg/mL,and that in the supernatant of Schwann cells in the BMSCs group was(109.70±6.38)pg/mL,with statistically significant difference(P<0.05).Conclusion Exosomes secreted by BMSCs can promote the proliferation,migration and the secretion of BDNF and VEGF of Schwann cells,thus contributing to nerve regeneration.
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