Expression levels of serum lncRNA H19 and LIPCAR in patients with AMI and their predictive efficacy for MACE
Objective To observe the changes in serum long non-coding RNA H19(lncRNA H19)and intergenic long non-coding RNA of cardiac remodeling(LIPCAR)levels in patients with acute myocardial infarction(AMI),and to analyze their predictive efficacy for major adverse cardiovascular events(MACE).Methods Totally 126 patients with AMI(observation group)and 60 healthy individuals who underwent physical examinations during the same period(control group)were selected.Real-time fluorescence quantitative PCR was used to detect serum lncRNA H19 and LIPCAR in both groups,and Pearson correlation analysis was used to analyze the relationships between serum lncRNA H19,LIPCAR and clinical parameters of AMI patients.Patients in the observation group were followed up for 6 months after percutaneous coronary intervention,and were divided into MACE patients(32 cases)and non-MACE patients(94 cases)based on whether MACE occurred.Logistic regression analysis was used to analyze the risk factors for MACE in AMI patients.Addi-tionally,the predictive value of serum lncRNA H19 and LIPCAR levels for MACE in AMI patients was analyzed using the receiver operating characteristic curve.Results Compared with the control group,the levels of serum lncRNA H19 and LIPCAR increased in the observation group(both P<0.05).The levels of serum lncRNA H19 and LIPCAR were related to LVEF(r=-0.752,-0.763,respectively;both P<0.05),the number of coronary artery lesions(r=0.672,0.731,re-spectively;both P<0.05),and Killip grading(r=0.667,0.712,respectively;both P<0.05)in AMI patients,but not to LVFS or CO(all P>0.05).Significant differences were found in the LIPCAR,lncRNA H19 levels,Killip grading,and LVEF between MACE and non-MACE individuals(all P<0.05).Serum LIPCAR,lncRNA H19 levels,and Killip grading were risk factors for MACE in AMI patients,while LVEF was a protective factor(all P<0.05).When the cut-off value of serum lncRNA H19 was 4.22,the area under the curve in predicting MACE of AMI patients was 0.877(0.835-0.926),the Youden index was 0.597,the sensitivity was 0.791,and the specificity was 0.806;when the cut-off value of serum LIPCAR was 5.47,the area under the curve in predicting MACE in AMI patients was 0.845(0.817-0.892),the Youden index was 0.581,the sensitivity was 0.757,and the specificity was 0.824;when we combined them,the area under the curve in predicting MACE in AMI patients was 0.921(0.889-0.941),the Youden index was 0.668,the sensitivity was 0.886,and the specificity was 0.782.Conclusions Serum lncRNA H19 and LIPCAR increase in AMI patients,which are related to cardiac function indicators and are risk factors for MACE in AMI patients.Combined detection can help pre-dict whether AMI patients will develop MACE.
acute myocardial infarctionlong non-coding RNA H19intergenic long non-coding RNA of cardiac re-modelingmain adverse cardiovascular events
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