摘要
目的 鉴定山慈菇的乙酸乙酯、石油醚、氯仿萃取物中的挥发性成分.方法 取3 kg山慈菇加工成粉末,分别以乙酸乙酯、石油醚、氯仿萃取,采用气相色谱—质谱联用法(GC-MS)对乙酸乙酯萃取物、石油醚萃取物、氯仿萃取物中挥发性成分进行分析与鉴定.结果 山慈菇乙酸乙酯萃取物中共鉴定出51种化合物,以儿茶酚含量最高,其次是4-羟基苯甲醛、2-乙基-3-甲基吡嗪、Phenol,4-(ethoxymethyl)-、肉桂酸、间羟基苯甲酸等;山慈菇石油醚萃取物共鉴定出35种化合物,包括肉桂酸、棕榈酸丁酯、十七酸甲酯、油酸、亚油酸等;山慈菇氯仿萃取物共鉴定出28种化合物,包括Phenol,4-(ethoxymethyl)-、2,6-二甲氧基苯酚、2-乙基-3-甲基吡嗪、肉桂酸等.结论 在山慈菇乙酸乙酯、石油醚、氯仿萃取物中抗癌挥发性成分都发现了肉桂酸,在山慈菇乙酸乙酯萃取物中发现了香草酸、间羟基苯甲酸、4-羟基苯甲醛等抗癌成分.山慈菇不同萃取物中首次发现儿茶酚、2-乙基-3-甲基吡嗪、Phenol,3-甲基环戊烷-1,2-二酮、甲基环戊烯醇酮、棕榈酸丁酯、十七酸甲酯、油酸、亚油酸、2,6-二甲氧基苯酚、2-乙基-3-甲基吡嗪等成分.
Abstract
Objective To identify the volatile ingredients in ethyl acetate,petroleum ether and chloroform extracts of Cremastra appendiculata.Methods Three kilograms of Cremastra appendiculata were processed into powder and ex-tracted with ethyl acetate,petroleum ether and chloroform,respectively.The volatile ingredients in ethyl acetate extract,petroleum ether extract and chloroform extract were analyzed and identified by gas chromatography-mass spectrometry(GC-MS).Results Fifty-one ingredients were identified from the ethyl acetate extract of Cremastra appendiculata,with the highest content of catechol,followed by 4-hydroxybenzaldehyde,2-ethyl-3-methylpyrazine,Phenol,4-(ethoxymethyl)-,cinnamic acid,m-hydroxybenzoic acid,etc.Thirty-five ingredients were identified from the petroleum ether extract of Cremastra appendiculata,including cinnamic acid,butyl palmitate,methyl heptadecanoate,oleic acid,linoleic acid,etc.Twenty-eight ingredients were identified from the chloroform extract of Cremastra appendiculata,including Phenol,4-(eth-oxymethyl)-,2,6-dimethoxyphenol,2-ethyl-3-methylpyrazine,cinnamic acid,etc.Conclusion Cinnamic acid was found in the volatile ingredients of ethyl acetate,petroleum ether and chloroform extracts of Cremastra appendiculata.Anti-cancer ingredients such as vanillic acid,m-hydroxybenzoic acid and 4-hydroxybenzaldehyde were found in the ethyl acetate extract of Cremastra appendiculata.Catechol,2-ethyl-3-methylpyrazine,Phenol,3-methylcyclopentane-1,2-dione,meth-ylcyclopentenolone,butyl palmitate,methyl heptadecanoate,oleic acid,linoleic acid,2,6-dimethoxyphenol,2-ethyl-3-methylpyrazine and other ingredients were found in different extracts of Cremastra appendiculata for the first time.
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