Determination of Uric Acid Based on Peroxidase-like Activity of Fe3O4@CeO2
A two-step hydrothermal method was employed to synthesize Fe3O4@CeO2 nanocomposites which has peroxidase-like activity.Fe3O4@CeO2 can catalyze the oxidation reaction of 3,3',5,5'-tetramethylbenzidine(TMB)by H2O2 to form a blue colored product(TMBox).A sensitive and selective colorimetric method for uric acid detection was established by cascading with the oxidation reaction of urieoxidase toward uric acid.The detection limit of uric acid was 7.9 μmol/L and the linear detection range was from 10 to 100 μmol/L.The test result was accurate and reliable.
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