Study on the Determination of Alkaloids in Essential Oil Cosmetics by High Performance Liquid Chromatography Tandem Mass Spectrometry
LC-MS/MS(high-performance liquid chromatography tandem mass spectrometry)technology was used to analyze 31 alkaloids in volatile oil cosmetics.In HPH-C18 column chromatography,a methanol water solution with a volume ratio of 2%formic acid(3:1)was used as the extraction solution.Gradient elution was performed with 0.2%formic acid water(mobile phase A)and acetonitrile(mobile phase B).The elution flow rate was controlled at 0.35 mL/min,and the injection amount was 5 μL.The multi reaction monitoring analysis was carried out through the positive ion mode of electric spray.The research results indicate that the linear relationship between the 31 alkaloids in essential oil cosmetics is relatively good within the mass concentration range,and the pre-treatment method is simple and efficient,which can effectively determine the 31 alkaloids in essential oil cosmetics and ensure the safety evaluation effect of cosmetics.
high performance liquid chromatography tandem mass spectrometryessential oil cosmeticsalkaloid
NETL
NSTL
万方数据
