基于适配体的纳米金比色法检测中药材中黄曲霉毒素B1
Colorimetric Determination of Aflatoxin B1 in Chinese Herbal Medicine based on Gold Nanoparticles and Aptamers
韩素平 1李晓红 2周水清 1白紫兰 1孙晓晶 1邢宪荣 1付正1
作者信息
- 1. 山东医学高等专科学校药学系,山东 济南 250002
- 2. 济南护理职业学院专业基础部,山东 济南 250102
- 折叠
摘要
中药材贮存加工过程中易受真菌毒素污染,其中黄曲霉毒素B1(AFB1)以其高毒性和致癌性备受关注.制备了金纳米粒子作为基质,采用核酸适配体作为特异性识别探针,构建了一种快速可视化检测中药材中AFB1 的方法.该方法检测AFB1 的线性范围为 2.5～100 ng/mL,检出限为 1.87 ng/mL.应用该方法测定麦芽、决明子、三七中AFB1的含量,加标回收率为89.1%～109.7%,相对标准偏差为 3.0%～8.3%.结果表明本方法具有良好的选择性和准确性.
Abstract
Chinese herbal medicine is susceptible to be contaminated by mycotoxins during the storage and processing.Among the mycotoxins,aflatoxin B1(AFB1)has attracted much attention due to its high toxicity and carcinogenicity.A rapid and visul detection method of AFB1 in Chinese herbal medicine was established.In this method,gold nanoparticles were employed as the matrix and nucleic acid aptamer was used as specific recognition probe.The detection limit of AFB1 was 1.87 ng/mL and the linear detection range was from 2.5 to 100 ng/mL.The established method was employed for the detection of AFB1 in malt,Semen Cassiae and Panax notoginseng.The spiked recovery tests of different samples revealed that the recovery rates were between 89.1%and 109.7%,and the relative standard deviation ranged from 3.0%to 8.3%.The results indicate that this method has good selectivity and accuracy.
关键词
纳米金/适配体/黄曲霉毒素B1/比色法Key words
gold nanoparticles/aptamer/aflatoxin B1/colorimetric detection引用本文复制引用
基金项目
山东省中医药科技项目(2021M219)
山东省中医药科技项目(M-2023253)
出版年
2024
NETL
NSTL
万方数据