Determination of Flufenicol API by High Performance Liquid Chromatography
This study established a liquid chromatography method for the determination of flufenicol.Determine the detection method by studying conditions such as chromatographic column separation,qualitative wavelength,and mobile phase selection.And evaluate the method through detection limit,quantification limit,recovery rate,and repeatability experiments.The results showed that TC-C18 column was selected as the separation column,with the optimal wavelength of 223 nm and the optimal mobile phase of 25%acetonitrile aqueous solution.The linearity of fluorophenicol reached 0.999 75,and the detection limit was 0.05 μg/mL,with a quantification limit of 0.15 μg/mL,with an addition amount of 0.5-3 μg.The recovery rate at g reaches 88.7%～100%,and the repeatability RSD reaches 0.64%.This method is efficient and accurate,and can provide reference for the determination of the main content of flufenicol,as well as ideas for the determination of other veterinary drugs such as flufenicol.
high performance liquid chromatographyflufenicolactive pharmaceutical ingredientsmain content
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