超声辅助低共熔溶剂提取蒲公英绿原酸的工艺优化及其抗氧化活性研究
Study on the Optimization of Ultrasonic-assisted Low Eutectic Solvent Solvent Extraction of Chlorogenic Acid from Dandelion and its Antioxidant Activity
卢娟 1韦新银 1谭春婷 1罗清淋 1王宏 1吴春燕1
作者信息
- 1. 广西民族师范学院化学与生物工程学院, 广西 崇左 532200
- 折叠
摘要
通过超声波辅助低共熔溶剂提取蒲公英中的绿原酸.考察了低共融溶剂摩尔比[n(氯化胆碱)∶n(柠檬酸)]、液料比、超声功率、提取温度、超声时间对蒲公英绿原酸得率的影响.在单因素实验的基础上,通过响应面实验优化得出提取蒲公英绿原酸的最佳工艺条件为:低共熔溶剂含水量 60%,n(氯化胆碱)∶n(柠檬酸)=1∶2,提取温度70℃,液料比35∶1(mL/g),超声功率 300 W,超声时间 42 min.在此条件下,蒲公英绿原酸的得率为 2.86%±0.05%,与预测值接近 2.89%接近,表明该模型可靠.同时对蒲公英绿原酸提取物抗氧化活性实验研究结果表明,蒲公英绿原酸的提取液在 1～6 μg/mL,DPPH自由基及ABTS+自由基的清除能力均随着浓度的增加而增大.在提取液质量浓度为6 μg/mL时,两者的清除率分别为82.9%和84.2%,说明蒲公英绿原酸提取物具有较强的抗氧化活性.
Abstract
Chlorogenic acid was extracted from dandelion by ultrasonic-assisted low eutectic solvent.The effects of low eutectic solvent molar ratio(choline chloride:citric acid),liquid-solid ratio,ultrasonic power,extraction temperature,and ultrasonic time on the yield of chlorogenic acid were investigated.Based on the single factor experiment,the optimal extraction conditions of chlorogenic acid from dandelion were obtained by response surface experiment:the low eutectic solvent with water content of 60%,the molar ratio of choline chloride and citric acid was 1:2,the extraction temperature was 70°C,the liquid-solid ratio was 35:1(mL/g),ultrasonic power was 300 W,and ultrasonic time was 42 min.Under these conditions,the yield of chlorogenic acid from dandelion was 2.86%±0.05%,which was close to the predicted value of 2.89%,indicating that the model was reliable.At the same time,the antioxidant activity of chlorogenic acid extract from dandelion was studied.The results showed that the extraction of chlorogenic acid from dandelion was in the range of 1～6μg/mL,and the scavenging ability of DPPH free radical and ABTS+ free radical increased with the increase of concentration.When the extraction concentration was 6 μg/mL,the scavenging rates of both were 82.9%and 84.2%,indicating that the chlorogenic acid extract from dandelion had strong antioxidant activity.
关键词
蒲公英/绿原酸/低共融溶剂/超声波/抗氧化活性Key words
dandelion/chlorogenic acid/low eutectic solvent/ultrasonic/antioxidant activity引用本文复制引用
基金项目
自治区级大学生创新创业训练计划训练立项项目(2023)(S202310604039)
广西民族师范学院科研项目人才科研启动基金(2020)(2020FG002)
广西民族师范学院科研项目人才科研启动基金(2020)(2020FG004)
出版年
2024
NETL
NSTL
万方数据