Study on the Optimization of Ultrasonic-assisted Low Eutectic Solvent Solvent Extraction of Chlorogenic Acid from Dandelion and its Antioxidant Activity
Chlorogenic acid was extracted from dandelion by ultrasonic-assisted low eutectic solvent.The effects of low eutectic solvent molar ratio(choline chloride:citric acid),liquid-solid ratio,ultrasonic power,extraction temperature,and ultrasonic time on the yield of chlorogenic acid were investigated.Based on the single factor experiment,the optimal extraction conditions of chlorogenic acid from dandelion were obtained by response surface experiment:the low eutectic solvent with water content of 60%,the molar ratio of choline chloride and citric acid was 1:2,the extraction temperature was 70°C,the liquid-solid ratio was 35:1(mL/g),ultrasonic power was 300 W,and ultrasonic time was 42 min.Under these conditions,the yield of chlorogenic acid from dandelion was 2.86%±0.05%,which was close to the predicted value of 2.89%,indicating that the model was reliable.At the same time,the antioxidant activity of chlorogenic acid extract from dandelion was studied.The results showed that the extraction of chlorogenic acid from dandelion was in the range of 1～6μg/mL,and the scavenging ability of DPPH free radical and ABTS+ free radical increased with the increase of concentration.When the extraction concentration was 6 μg/mL,the scavenging rates of both were 82.9%and 84.2%,indicating that the chlorogenic acid extract from dandelion had strong antioxidant activity.
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