Optimization of Extraction Process of Luteolin from Peanut Shells Based on Molecular Imprinted Electrochemical method
Molecularly imprinted polymer(MIP)was prepared by cyclic voltammetry(CV)in-situ polymerization with p-aminophenol(p-AP)and 3,4-ethylenedioxythiophene(EDOT)as bifunctional monomers and Luteolin as template molecules for the construction of electrochemical sensors.Enzymatic hydrolysis-assisted ultrasonic method was used to extract luteolin from peanut shells,and the extraction process was optimized by Box-Behnken response surface method.The results showed that p-AP and EDOT achieved CV copolymerization on the electrode surface,and MIP showed good electrocatalysis and recognition ability for luteolin.The optimal extraction process was as follows:enzymatic hydrolysis time was 81 min,enzymatic hydrolysis temperature was 45℃,ultrasonic time was 60 min,ultrasonic temperature was 45℃,and the extraction yield of Luteolin reached 0.862 0%(RSD%=2.525%),which was close to that obtained by HPLC method(0.892 2%),indicating that the established detection method was accurate and reliable.
NETL
NSTL
万方数据
