Content Determination of Polysaccharides of Ganoderma By Phenol-Sulfuric Acid Method
Objective:To investigate the applicability of UV-vis spectrophotometry for the determination of polysaccharide in Ganoderma lucidum mycelium.Methods:The contents of polysaccharide in ganoderma lucidum mycelia were determined at 485 nm with anhydrous glucose as reference substance,phenol and sulfuric acid as chromogenic agent.Results:The polysaccharide in ganoderma lucidum showed a good relationship with in the range of 0～0.115 20 mg(R2=0.999 2),precision RSD was 1.42%.The sample solution has good stability within 12 hour after preparation,the average recovery rate was 100.61%,RSD was 0.97%.Conclusion:The method was convenient and fast.It has good reproducibility and reliable results.It can be used to determine the content of polysaccharide in ganoderma lucidum mycelium.
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