苯酚-硫酸法测定灵芝菌丝多糖含量的研究
Content Determination of Polysaccharides of Ganoderma By Phenol-Sulfuric Acid Method
孙国强 1董金颖 1董建生1
作者信息
- 1. 山西瑞芝生物科技有限公司, 山西 运城 044000
- 折叠
摘要
目的:建立紫外-可见分光光度法测定灵芝菌丝中多糖含量的方法.方法:以无水葡萄糖为对照品,苯酚和硫酸为显色剂,在 485nm波长处测定灵芝菌丝体中多糖含量.结果:灵芝菌丝中多糖含量0～0.115 20 mg范围内线性关系良好(R2=0.999 2),精密度RSD为 1.42%.样品溶液在配制后 12h内稳定性较好,平均加标回收率为100.61%,RSD为0.97%.结论:该方法方便快捷,重现性好,结果可靠,适用于测定灵芝菌丝体中多糖含量.
Abstract
Objective:To investigate the applicability of UV-vis spectrophotometry for the determination of polysaccharide in Ganoderma lucidum mycelium.Methods:The contents of polysaccharide in ganoderma lucidum mycelia were determined at 485 nm with anhydrous glucose as reference substance,phenol and sulfuric acid as chromogenic agent.Results:The polysaccharide in ganoderma lucidum showed a good relationship with in the range of 0～0.115 20 mg(R2=0.999 2),precision RSD was 1.42%.The sample solution has good stability within 12 hour after preparation,the average recovery rate was 100.61%,RSD was 0.97%.Conclusion:The method was convenient and fast.It has good reproducibility and reliable results.It can be used to determine the content of polysaccharide in ganoderma lucidum mycelium.
关键词
灵芝菌丝/多糖含量/苯酚-硫酸法Key words
Ganoderma lucidum mycelium/polysaccharide/phenol-sulfuric acid method引用本文复制引用
出版年
2024
NETL
NSTL
万方数据