忍冬叶总酚的提取及其抗氧化活性研究
Extraction and Antioxidant Activity of Total Phenols From Leaves of Lonicera japonica Thunb
张冬 1贾莲2
作者信息
- 1. 北方测盟测试技术(辽宁)有限公司,辽宁 沈阳 110042
- 2. 鞍山师范学院化学与生命科学学院,辽宁 鞍山 114007;辽宁省天然产物活性分子开发及利用重点实验室,辽宁 鞍山 114007
- 折叠
摘要
总酚是忍冬叶中主要的活性成分.本研究以忍冬叶为材料,采用乙醇为溶剂,结合超声波辅助法提取总酚,考察乙醇浓度、料液比和提取时间 3 个单因素对忍冬叶总酚得率的影响,利用正交实验对提取工艺进行优化,同时通过忍冬叶总酚对 1,1-二苯基-2-三硝基苯肼(DPPH·)清除能力来评价其抗氧化活性.结果表明,最佳的工艺条件为乙醇体积分数70%、料液比 1∶60、超声时间30 min时.在此条件下忍冬叶总酚提取率最高,为9.18 mg/g,忍冬叶提取物对DPPH·自由基清除率达到 90.79%,具有较强的抗氧化能力.
Abstract
Total phenols are the main active ingredient in Lonicera japonica leaves.This study used L.japonica leaves as materials,ethanol as a solvent combined with ultrasonic assisted extraction of total phenols.The influence of three single factors of ethanol concentration,solid-liquid ratio,and extraction time on the extraction yield of total phenols from leaves of L.japonica was investigated.The technological factors affecting the extraction yield of total phenols from leaves of L.japonica was optimized by orthogonal method.The antioxidant activity was evaluated through the scavenging effect of total phenols from leaves of L.japonica on 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH·).Results showed that the optimal conditions of extracting total phenol from leaves of L.Japonica was ethanol concentration 70%,solid/liquid ratio 1:60 and extracting 30 min.Under these conditions the yield of total phenols was 9.18 mg/g.At this time,the DPPH·free radical scavenging rate of the total phenols from leaves of L.Japonica reached 90.79%,indicating strong antioxidant ability.
关键词
忍冬/总酚/超声提取/工艺优化/抗氧化活性/DPPHKey words
Lonicera japonica/total phenol/ultrsound assisted/process optimization/antioxidant activity/DPPH引用本文复制引用
基金项目
辽宁省自然科学基金资助计划项目(2019-MS-001)
辽宁省教育厅青年项目(扶持项目)(jytqnfc202103)
出版年
2024
NETL
NSTL
万方数据