Determination of α-solanine and α-chaconine in Poisoned Blood by UPLC-MS/MS
To establish a method for the determination of α-solanine and α-chaconine in blood by ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS).The target compounds were extracted by acetonitrile precipitation protein method,and analyzed by multiple reaction monitoring(MRM)under positive ion mode(ESI+)of the electrospray ion source.The results show that the detection limits of α-solanine and α-chaconine in blood were 0.10 ng/mL and 0.11 ng/mL,respectively.The linear relationship was good in the range of 0.5～50 ng/mL,and R2 was greater than 0.998.In the low,medium and high(0.5 ng/mL,5 ng/mL,50 ng/mL)concentrations,the matrix effect was between 92.64%and 110.45%,the extraction recovery was between 89.23%and 105.08%,and the intra-day and inter-day precision RSD was not above 5.94%.The method has good specificity,high sensitivity and good accuracy,and can be used for rapid detection of α-solanine and α-chaconine in blood.
α-solanineα-chaconinepoisoningbloodultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)
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