Explore and optimize the process of separating and purifying total flavonoids from Artemisia argyi using macroporous resin.This experiment uses a single factor experiment to optimize the separation and purification process.The results demonstrate that HPD100 macroporous resin has the most impressive adsorption and desorption capability.The ideal process conditions are:loading time of 90 minutes,pH of the solution at 3,adsorption temperature of 40℃,concentration of the solution at 0.2 mg/mL,flow rate of 1.5 mL/min,and adsorption rate of 68.5%.Elution was conducted with 80%ethanol by volume,with a flow rate of 1.5 mL/min and elution volume of 100 mL,resulting in a high elution rate of 60.45%.The purification effect was evidently effective,as the purity of total flavonoids in Artemisia argyi rose from 5.9%to 13.5%under these adsorption and desorption conditions.
mugwortflavonoidsmacroporous resinseparation and purification process
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