Study on the Separation Technology of Total Flavonoids from Moxa Wool by Macroporous Resin
Explore and optimize the process of separating and purifying total flavonoids from Artemisia argyi using macroporous resin.This experiment uses a single factor experiment to optimize the separation and purification process.The results demonstrate that HPD100 macroporous resin has the most impressive adsorption and desorption capability.The ideal process conditions are:loading time of 90 minutes,pH of the solution at 3,adsorption temperature of 40℃,concentration of the solution at 0.2 mg/mL,flow rate of 1.5 mL/min,and adsorption rate of 68.5%.Elution was conducted with 80%ethanol by volume,with a flow rate of 1.5 mL/min and elution volume of 100 mL,resulting in a high elution rate of 60.45%.The purification effect was evidently effective,as the purity of total flavonoids in Artemisia argyi rose from 5.9%to 13.5%under these adsorption and desorption conditions.
mugwortflavonoidsmacroporous resinseparation and purification process
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