大孔树脂分离纯化艾绒总黄酮工艺探索
Study on the Separation Technology of Total Flavonoids from Moxa Wool by Macroporous Resin
常孜伊 1张旭霞 1侯乐 1刘天金 1呼楠 1李文科 1宋建庆 1张博文 1毛伟桓 1周奕楷 1梁燕 1朱江敏 1王慧芳1
作者信息
- 1. 太原工业学院化学与化工系,山西 太原 030008
- 折叠
摘要
探索优化大孔树脂分离纯化艾绒总黄酮的工艺.本实验采用单因素实验优化分离纯化工艺.经过研究,发现HPD100大孔树脂具有最优的吸附和解析附能力.采用90 min的上样时间,pH=3,吸附温度 40℃,上样液质量浓度0.2 mg/mL,上样液流速 1.5 mL/min,吸附率可达到 68.5%.用体积分数 80%乙醇洗脱,流速为1.5 mL/min,洗脱液用量为100 mL,洗脱率高达到 60.45%.在此吸附和解析条件下,艾绒总黄酮的纯度由 5.9%提升到 13.5%,提纯效果好.
Abstract
Explore and optimize the process of separating and purifying total flavonoids from Artemisia argyi using macroporous resin.This experiment uses a single factor experiment to optimize the separation and purification process.The results demonstrate that HPD100 macroporous resin has the most impressive adsorption and desorption capability.The ideal process conditions are:loading time of 90 minutes,pH of the solution at 3,adsorption temperature of 40℃,concentration of the solution at 0.2 mg/mL,flow rate of 1.5 mL/min,and adsorption rate of 68.5%.Elution was conducted with 80%ethanol by volume,with a flow rate of 1.5 mL/min and elution volume of 100 mL,resulting in a high elution rate of 60.45%.The purification effect was evidently effective,as the purity of total flavonoids in Artemisia argyi rose from 5.9%to 13.5%under these adsorption and desorption conditions.
关键词
艾绒/黄酮/大孔树脂/分离纯化工艺Key words
mugwort/flavonoids/macroporous resin/separation and purification process引用本文复制引用
基金项目
山西省大学生创新创业训练项目(S202314101094)
太原工业学院大学生创新创业训练项目(GK2023062)
出版年
2024
NETL
NSTL
万方数据