Screening Study one DNA Macro-barcoding of Benthic Animals in a Freshwater Basin
Due to the advantages of eDNA macro-barcoding technology,such as fast and accurate,it has become a research hotspot in recent years to utilize this method to investigate the diversity of benthic fauna in freshwater basins and to evaluate the aquatic ecological health.In this study,we calculated and compared the evolutionary rates and gene differential sites of cox1,cob,12SrDNA and 18SrDNA genes,and the results showed that the cox1 gene has moderate evolutionary rate and high interspecific differences,which is suitable for use as a universal gene for eDNA macro barcoding.By comparing the effects of different lengths of cox1 gene fragments on the results,it was found that short fragments around 300bp had higher coverage in terms of benthic species diversity.Therefore,this study proposes to use 300bp length cox1 sequence as a universal gene fragment for eDNA macro barcoding of freshwater benthos in order to improve the coverage of species diversity assessment and to provide technical services for biodiversity research and ecological restoration in freshwater ecosystems.
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