Research on Infrared Spectral Recognition of the Venom of Golden Hornet from Different Regions
Fourier transform infrared spectroscopy(FTIR)combined with second derivative infrared spectroscopy was used to analyze and identify the venom of five different origins of golden ring wasps.The results showed that the one-dimensional infrared spectra of wasp venom from different origins exhibited characteristic absorption of proteins,amino acids,polysaccharides,alkaloids,and aromatic compounds at 1 659～1 658 cm-1,1 545～1 539 cm-1,1 467～1 455 cm-1,1 100～1 041 cm-1,and other locations.However,due to different origins,there are certain differences in the position and intensity of the absorption peaks of each sample.In addition,the absorption peaks in the ranges of 1 467～1 455 cm-1,1 386～1 368 cm-1,and 1 100～1 041 cm-1 are the main characteristic peaks for identifying different bee venom samples.Comparing the second derivative infrared spectra of wasp venom from different regions,it was found that the absorption peaks near 1 512 and 1 500 cm-1 further confirmed the presence of aromatic compounds in wasp venom.In addition,there were significant differences in the intensity and position of the absorption peaks near 1 712 cm-1 and 1 500 cm-1,as well as the absorption peak in the range of 1700 to 1 620 cm-1 among the samples.The application of infrared spectroscopy and second derivative infrared spectroscopy can not only provide relevant information on the main chemical components of the venom of the golden ring wasp,but also effectively distinguish the venom of the golden ring wasp from different origins.This method is fast,intuitive,and simple,and can provide a large amount of information on the overall chemical composition of wasp venom from different origins,which is helpful for the overall quality control and qualitative analysis of effective ingredients in wasp venom.
golden ring waspbee venomplace of originfourier transform infrared spectroscopy
万方数据
维普
