Study on Powder Microscopic Characteristics and Thin Layer Chromatography Identification of Capsella Bursa-pastoris(L.)Medic
Objective:In order to improve the quality standard of Capsella bursa-pastoris(L.)Medic.,the powder microscopic characteristics and thin layer chromatography(TLC)identification methods were studied.Methods:The powder microscopic characteristics of Capsella bursa-pastoris(L.)Medic.were studied by optical microscope.Leucine,valine and Capsella bursa-pastoris(L.)Medic.were used as the reference materials in TLC identification.Results:The characteristics of microscopic identification were obvious,among them non-glandular hair and stomata can be used as the main distinguishing points of Capsella bursa-pastoris(L.)Medic.The parameters were determined as follows:silica gel G thin-layer plate,dilute ethanol was used as the extraction solvent,ultrasound for 20 minutes,n-butanol-glacial acetic acid-water(4:1:1)as the development agent,ninhydrin as chromogenic agent,and heating at 105℃until the spots showed clear color in the daylight.The chmmatographic spots were clear,without intererence and easy to recognize.Conclusion:The method is stable,simple,feasible and reproducible.It can provide experimental basis for powder microscopic characteristics and TLC identification of Capsella bursa-pastoris(L.)Medic.
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