Determination of Etomidate Content in Electronic Cigarette oil by HPLC-MS/MS Method
OBJECTIVE:To establish an HPLC-MS/MS method for simultaneous determination of illegally added etomidate in electronic cigarette oil.METHOD:Analysis using WATERS CORTECS UPLC T3 column(2.1×100 mm,1.6 μm),mobile phase acetonitrile(A)-0.1%formic acid aqueous solution(B),gradient elution mode,chromatographic column temperature at 40℃,injection volume of 1 μL.Electrospray ion source(ESI),positive ion scanning mode,monitoring mode:FULL MS.RESULTS:Etomidate has a good linear relationship in the range of 1.02～102.39 ng/ml,r=0.999 5,the average recovery rate is 99.62%,and the RSD is 0.37%.CONCLUSION:The results of this method are accurate and reliable,eliminating possible interference caused by identification methods such as UV spectroscopy,liquid chromatography,and infrared spectroscopy.It can be used for the determination of illegal addition of etomidate in electronic cigarette oil.
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