QuEChERS-高效液相色谱/串联质谱法测定药食同源中药材中30种农药残留
Determination of 30 Pesticide Residues in Chinese Medicinal Materials with Homology of Medicine and Food by QuEChERS-High Performance Liquid Chromatography Tandem Mass Spectrometry
秦晋钰 1魏嘉波2
作者信息
- 1. 山西药科职业学院,山西 太原 030031
- 2. 大同市综合检验检测中心,山西 大同 037000
- 折叠
摘要
目的:采用QuEChERS结合高效液相色谱/串联质谱法建立了蒲公英、甘草、当归、党参、黄芪 5 种中药材中30 种农药残留的快速分析方法.方法:样品用乙腈均质提取,经无水硫酸钠-PSA柱吸附净化,在MRM模式下进行质谱分析,外标法定量.结果:30种农药在 1.0～100 ng/mL范围内具有良好的线性关系,相关系数均大于 0.996.30 种农药的平均加标回收率为 90.3%～110%,RSD为 0.9%～20%.结论:本方法灵敏、准确、高效,可用于同时进行药食同源中药材中 30种农药残留的快速筛查与检测,为药食同源中药材的种植生产和质量管控提供数据参考.
Abstract
Objective:To establish a rapid analysis method for 30 pesticide residues in five chinese medicinal materials with homology of medicine and food,including dandelion,licorice,angelica sinensis,codonopsis pilosula,and astragalus membranaceus,using QuEChERS combined with high-performance liquid chromatography tandem mass spectrometry.Method:The sample was homogenized and extracted with acetonitrile,purified by anhydrous sodium sulfate PSA column adsorption,and analyzed by mass spectrometry in MRM mode using external standard method for quantification.Results:The 30 pesticides showed a good linear relationship within the range of 1.0～100 ng/mL,with correlation coefficients greater than 0.996.The average spiked recovery rate of 30 pesticides is 90.3%to 110%,with RSD ranging from 0.9%to 20%.Conclusion:This method is sensitive,accurate,and efficient,and can be used for rapid screening and detection of 30 pesticide residues in five chinese medicinal materials with homology of medicine and food.It provides data reference for the cultivation,production,and quality control of medicinal and edible Chinese medicinal materials.
关键词
QuEChERS/高效液相色谱-串联质谱法/药食同源/农药残留Key words
QuEChERS/high performance liquid chromatography tandem mass spectrometry/homology of medicine and food/pesticide residues引用本文复制引用
出版年
2024
NETL
NSTL
万方数据