Determination of Tetrandrine Content in Refined Dog Skin Cream
Objective:To establish a method for detecting the content of tetrandrine in refined dog skin ointment and verify the analytical method.Method:GL Sciences InertSustain C18 4.6×250 mm×5 μm chromatography column(manufacturer's article number:5020-07346).Using methanol as the mobile phase A,acetonitrile as the mobile phase B,and water(adding 0.41 g of sodium dodecyl sulfonate and 0.1 mL of glacial acetic acid every 30 mL)as the mobile phase C,gradient elution is carried out.Flow rate of 1.5mL/min.Column temperature of 30℃.The detection wavelength is 280 nm.Result:A linear equation was obtained:A=76.227*C-0.0078(r=1),with a good linear relationship.The recovery rates of each group ranged from 92.48%to 105.73%,and the RSD between the recovery rates of each group was≤4.0%.Conclusion:The proposed method can be used for the determination of tetrandrine content in refined dog skin ointment,gel,and tablets.
refined dog skin ointmenttetrandrinecontent determination
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