Research on the Extraction Process and Antioxidant Activity of Total Flavonoids from Fructus Aurantii
Objective:To optimize the optimal extraction process of total flavonoids in Fructus Aurantii and conduct antioxidant research on it.Method:Using rutin as the standard,the extraction rate of total flavonoids from Fructus Aurantii was determined by UV spectrophotometry.Determination of antioxidant capacity of total flavonoids in Fructus Aurantii immaturus using DPPH radical scavenging assay.Result:The optimal extraction process for total flavonoids in Fructus Aurantii was selected as follows:70％ethanol solution was used as the extraction solution,the solid-liquid ratio was 1∶10,and the extraction was carried out by reflux extraction at 80 ℃ for 1.5 hours.The extraction rate of total flavonoids in Fructus Aurantii could reach 15.09％.The scavenging rate of total flavonoids extracted from Fructus Aurantii on DPPH free radicals is positively correlated with its concentration.Conclusion:The total flavonoids in Fructus Aurantii have antioxidant activity.
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