Study on the HaCaT cells by total glucosides of paeonia lactiflora through the JAK/STAT3/BCL-XL signaling pathway
Objective:IL-22 was applied to stir the HaCaT cells to construct psoriasis model and to detect the changes of anti-apoptotic signaling molecule in the model.So in this study,total glucosides of paeony(TGP)was used to treat the cell model to explore the possible therapeutic mechanism for psoriasis.Methods:After HaCaT cells were stimulated with different concentrations of IL-22 for different periods of time,the changes of cell activity were detec-ted by MTT,and the optimal parameters of IL-22 were determined.RT-PCR and WB were used to detect the expres-sion of inflammatory and apoptosis-related molecules in IL-22 induced psoriatic cell models.The MTT and the Im-munofluorescence staining were applied to measure cell activity of psoriasis cell model after treated with TGP.RT-PCR and WB were used to detect the expression of downstream anti-apoptotic molecule BCL-XL after inhibition of STAT3 expression.The experiment was randomly grouped,and Graph Pad-Prism 6 software was used to analyze the results by t test.Results:MTT results showed that the optimal concentration of IL-22 in HaCaT cells was 12.5 mmol/L and the optimal treatment time was 48 h.The expression levels of inflammatory and apoptosis-related molecular signaling pathway J AK/STAT3/BCL-XL were significantly increased in IL-22-induced psoriasis cell model.The activity of Psoriatic cell model was decreased after treated with TGP,as same the localization of STAT3 in nucleus.Inhibition of STAT3 expression significantly reduced the activity of psoriatic cell model and the expression of STAT3 and its downstream anti-apoptotic molecule BCL-XL.Conclusion:TGP could reduce the activity of IL-22 induced psoriasis cell model by inhibiting the expression and activation of inflammatory and apoptotic signaling cascade JAK/STAT3/BCL-XL.
PsoriasisTotal glucosides of paeonyHaCaTResistance to apoptosisSTAT3BCL-XL
万方数据
维普
