Effects of Jiedu Huoxue decoction on CKD rat kidney Smad2,Smad3
Objective:To observe the effect of Jiedu Huoxue decoction on chronic kidney disease(CKD)by regu-lating the expression of Smads protein in kidney tissues of rats with CKD.Methods:Seventy-two male SD rats were randomly divided into six groups,the blank group,the model group,the positive control group,the low-dose group of traditional Chinese medicine,the medium-dose group of traditional Chinese medicine,and the high-dose group of tra-ditional Chinese medicine,with 12 rats in each group.Except for the blank group,the rats in the other groups were gavaged with adenine suspension and fed a high-phosphorus diet to establish a CKD rat model,and the rats were trea-ted with the drug by gavage for a period of 12 weeks after the modeling period of 8 weeks.On the 12th weekend,bio-chemical tests were conducted on rat blood samples for creatinine(Scr),urea nitrogen(BUN),phosphorus(P),blood calcium(Ca).On the 12th weekend,expression of Smad2 and Smad3 proteins in the kidney tissues.Results:Comparison of data between groups was made after completing the whole process of drug administration and analy-sis.Compared with the blank group,the serum levels of Scr,BUN,P and Ca in the remaining groups of rats were sig-nificantly different(P＜0.01 or P＜0.05);compared with the model group,the serum levels of Scr,BUN and P in the remaining groups of rats were generally decreased(P＜0.05 or P＜0.05),while Ca levels were increased(P＜0.05).Compared with the blank group,the remaining groups of The expression of Smad2 and Smad3 in rat kidney tissues was significantly increased(P＜0.05).Compared with the model group,the expression of Smad2 and Smad3 in the kidney tissues of the rest of the groups were decreased(P＜0.05).Compared with the low-dose group of de-toxification and activation of blood circulation soup,the expression of Smad2 protein and Smad3 protein in the high-dose group,middle-dose group and positive control group were further decreased(P＜0.05).Conclusion:Jiedu Huox-ue decoction shows a positive effect on delaying the process of CKD and effectively elevated the expression of Smad2 and decreased Smad3 in renal tissues.Compared with the rest of the medication groups,the high dose group of Jiedu Huoxue decoction demonstrated more significant efficacy,and the results of the study showed that there is a positive correla-tion between the drug dose and the efficacy.
Chronic kidney diseaseJiedu Huoxue decoctionRatsQuwan ChencuoImmunohistochemical methodSmad2 proteinSmad3 protein
万方数据
维普
