The mechanism of mitochondrial regulation by Jiao Ai decoction in a model of idiopathic miscarriage
Objective:To explore the mechanism of mitochondrial regulation by Jiao Ai decoction in a model of idiopathic miscarriage.Methods:Cell experiments and animal experiments were conducted.In the cell experiment,the control group,the Jiao Ai decoction group,the miR-29α-3p mimic group,and the Jiao Ai decoction+miR-29α-3p mimic group were set up.Human chorionic trophoblast cells HTR-8/SVneo were cultured and treated,and the ex-pression of miR-29α-3p,mitochondrial apoptosis,cytochrome C release,apoptosis-related protein expression,cell mi-gration and invasion ability were detected.In the animal experiment,pregnant C57BL/6J mice were selected to estab-lish a miscarriage model,and the groups were treated with Jiao Ai decoction by gavage and miR-29α-3p mimic by in-traperitoneal injection.The miscarriage situation,placental histology changes,miR-29α-3p expression,mitochondrial apoptosis-related indicators,and oxidative stress indicators were observed.Results:In the cell experiment,compared with the control group,the expression level of miR-29α-3p in the Jiao Ai decoction group was significantly increased at 48 hours(P＜0.001),the expression level in the miR-29α-3p mimic group was also significantly higher than that in the control group,and the expression level in the Jiao Ai decoction+miR-29α-3p mimic group was the highest.Jiao Ai decoction and miR-29α-3p mimic could reduce the change of mitochondrial membrane potential,reduce the release of cytochrome C,regulate the expression of apoptosis-related proteins(Bax expression decreased,Bcl-2 expression in-creased,Caspase-3 activity decreased),and make them shift to the anti-apoptotic direction,thereby inhibiting mito-chondrial apoptosis,and the combined use had a better effect.Jiao Ai decoction and miR-29α-3p mimic could also pro-mote the migration and invasion ability of cells,and the combined use had a more significant effect.In the animal ex-periment,the miscarriage rate in the miscarriage model group was higher,at(31.17±0.67)％.The miscarriage rate in the Jiao Ai decoction treatment group was reduced to(13.30±0.36)％.The miscarriage rate in the Jiao Ai decoc-tion+miR-29α-3p mimic group was further reduced to(5.77±0.36)％(P＜0.001).The morphology and structure of placental tissue in the Jiao Ai decoction treatment group were improved,and the damage of trophoblast cells was reduced.The degree of damage to placental tissue in the Jiao Ai decoction+miR-29α-3p mimic group was lighter,and the morphology of trophoblast cells was basically normal.The expression level of miR-29α-3p in placental tissue was increased in the Jiao Ai decoction treatment group and the Jiao Ai decoction+miR-29α-3p mimic group,and the ex-pression level in the Jiao Ai decoction+miR-29α-3p mimic group was higher.In the placental tissue of the Jiao Ai de-coction treatment group and the Jiao Ai Decoction+miR-29α-3p mimic group,Bax expression was decreased(P＜0.01),Bcl-2 expression was increased(P＜0.001),and Caspase-3 activity was decreased(P＜0.01).In the placental tissue of the Jiao Ai decoction treatment group and the Jiao Ai decoction+miR-29α-3p mimic group,SOD activity was increased,MDA content was decreased,and the improvement effect of the Jiao Ai decoction+miR-29α-3p mimic group was better(P＜0.001).Conclusion:J iao Ai decoction can up-regulate the expression of miR-29α-3p,regulate mitochondrial apoptosis,intervene in the invasion and migration of trophoblast cells,and reduce the risk of miscar-riage.miR-29α-3p plays an important role in it.
Spontaneous abortionJiao Ai decoctionmiR-29α-3pMitochondrialApoptosisTrophoblast cell
万方数据
维普
