首页|麻疯树硬酯酰-酰基载体蛋白脱饱和酶基因的克隆及特征

麻疯树硬酯酰-酰基载体蛋白脱饱和酶基因的克隆及特征

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
Cloning and characterization of a stearoyl-ACP desaturase gene from Jatropha curcas
Using degenerate primers and RT-PCR, RACE techniques, a 1491 bp cDNA segment of stearoyl-acyl carrier protein desaturase (SAD) is cloned from developing seeds of Jatropha curcas L. The segment contains a 1191 bp of complete open reading frame (ORF). Analysis in the BLAST on NCBI shows that Jatropha curcas SAD (JSAD) gene encodes a protein precursor composed of a signal peptide of 33 amino acids and a mature peptide of 363 amino acids. The homological analysis shows that JSAD has high level of homology both in nucleotide sequence and in amino acid sequence to other plants SADs. The nucleotide and peptide identity of JSAD to Ricinus communis SAD (RSAD) is up to 89% and 96.2% respectively. Molecular modeling of JSAD indicates that its three-dimensional structure strongly resembled the crystal structure of RSAD.

Jatropha curcasstearoyl-acyl carrier protein desaturasefatty acidgene cloningbioinformatics analysis

罗通、马丹炜、徐莺、邓骛远、肖猛、卿人韦、陈放

展开 >

College of Life Sciences, Sichuan University, Chengdu 610064, P. R. China

Department of Bioengineering, Yibin University, Yibin 64t007, P. R. China

Jatropha curcas stearoyl-acyl carrier protein desaturase fatty acid gene cloning bioinformatics analysis

Tenth Five Years Key Program of the State Science and Technology Commission in ChinaTenth Five Years Key Program of the State Science and Technology Commission in China

2002BA901A152004BA411B01

2007

上海大学学报(英文版)
上海大学

上海大学学报(英文版)

影响因子：0.196
ISSN：1007-6417
年,卷(期)：2007.11(2)
  • 5
  • 1