首页|水稻颖壳异常发育突变体Oryza sativa extraordinary glume 1(Oseg 1)的遗传与基因初定位分析

水稻颖壳异常发育突变体Oryza sativa extraordinary glume 1(Oseg 1)的遗传与基因初定位分析

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Phenotypic characterization of a rice mutant Oryza sativa extraordinary glume 1 (Oseg 1) and its genetic analysis
A rice mutant with Japonica 9522 cultivar background Oryza sativa extraordinary glume 1 (Oseg 1) was identified from the M2 mutant pool mutagenized by 60Co γ-ray. Compared with wild type plants, Oseg 1 developed longer empty glumes and rudimentary glumes. In some Oseg 1 mutants, the number of stamens of flowers was reduced and leaf-like lodicules occurred, and excessive lemma/palea-like organ could be observed in some mutant spikelets. This indicated that OsEG1 could regulate the development of rudimentary glumes, empty glumes, lemma/palea, lodicules, and stamens. Genetic analysis indicated that Oseg 1 came from a single recessive genetic locus. To clone OsEG1 gene, F2 population was constructed by a cross between Oseg 1 (Japonica) and Guangluai4 (Indica). Using map-based cloning approach, OsEG1 was mapped on chromosome 4, between INDEL marker OS407 and WHM0466 with genetic distance of 2.0 cm and 1.0 cm, respectively. These results are useful for further cloning and functional analysis of the OsEG1 gene.

rice (Oryza sativa L.), glume, OsEG1, spikelet meristem, primary mapping.

王红梅、储黄伟、刘海生、李晓星、杨功达、张大兵、雍克岚

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School of Life Sciences, Shanghai University, Shanghai 200444, P. R. China

SJTU-SIBS-PSU Joint Center for Life Sciences, School of Life Science and Biotechnology, Shanghai Jiaotong University,Shanghai 200240, P. R. China

rice (Oryza sativa L.), glume, OsEG1, spikelet meristem, primary mapping.

科技部科研项目科技部科研项目国家高技术研究发展计划(863计划)上海市自然科学基金上海市自然科学基金教育部跨世纪优秀人才培养计划上海市教育厅科研基金

2001CB1090022005CB1208022005AA271033003JC1406103DJ14016NCET-04-040304SG15

2007

上海大学学报(英文版)
上海大学

上海大学学报(英文版)

影响因子:0.196
ISSN:1007-6417
年,卷(期):2007.11(6)
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