Lipases in the castor bean seed of Chinese varieties: Activity comparison,purification and characterization

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外文摘要：The lipase activities in the three main Chinese castor bean varieties[ZiBi 05(ZB 5),TongBi 06(TB 6),JinBi 02(JB 2)]were investigated.Two lipases were found in extracts from the endosperms of all the three varieties.One lipase is located in the lipid bodies.showing higher activity at pH 4.5 and exhibiting the highest activity at day 1 of germination.The other lipase is located in the particulates.showing higher activity at pH 9.5 and particularly active at day 4 of germination.The lipid bodies from ZB 5 show the highest lipase activity at day 1 and pH 4.5 among the three varieties during the 8 d of germination.The acid lipase located in the lipid bodies of ZB 5 of germination 1 d Was purified to homogeneity by DEAE-sepharose CL-6B and butyl-sepharose CL-4B chromatography after isolation,delipidation and solubilization,with a purification factor of 16.1 and overall activity recovery of 17.7%.The characterizations(such as optimum pH,pH stability,optimum temperature,thermal stability,effect of metal ions,organic solvents stability,substrate specificity)of the purified acid lipase were conducted in detail.A combination of the characterized properties may make this enzyme a useful biocatalyst for industrial application.

外文关键词：

castor beanacid lipaselipid bodyparticulategermination

作者：

SU Er-zheng、ZHOU Ying、YOU Peng-yong、WEI Dong-zhi

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作者单位：

State Key Laboratory of Bioreactor Engineering,New World Institute of Biotechnology,East China University of Science and Technology,Shanghai 200237,P.R.China

基金：

Key Laboratory of Renewable Energy and Gas HydShanghai Postdoctoral Scientific Program中国博士后科学基金National Special Foundation for State Key Labo

项目编号：

KLREGH0807k409R21421400200904506702060204

出版年：

2010

DOI：

10.1007/s11741-010-0212-1

上海大学学报(英文版)

上海大学

上海大学学报(英文版)

影响因子：0.196

ISSN：1007-6417

年,卷(期)：2010.14(2)

参考文献量30