CO2 fixation influencing factors and synergistic strategies by non-photosynthetic autotrophic microorganisms
Microbial CO2 fixation is a promising pathway of carbon capture and utiliza-tion,which is crucial for achieving carbon neutrality in global ecosystems.Non-photosynthetic autotrophic microorganisms have attracted attention for their environmental adaptability,diversity of carbon fixation pathways,and production of high value-added products.Based on domestic and foreign researchs on CO2 fixation by non-photosynthetic autotrophic mi-croorganisms,we systematically analyzed internal and external factors affecting the carbon fixation efficiency of non-photosynthetic autotrophic microorganisms,including CO2 fixa-tion pathways,key carbon fixing enzymes,carbon sources,electron donors,and metabo-lites.Various strategies for promoting microbial CO2 fixation have been proposed.In addition to strategies involving direct responses to influencing factors,strategies involv-ing synergy between microorganisms and the use of metabolic promoters(biochar and micro/nanobubbles)are also being investigated.This review provides a theoretical foun-dation and technical support for enhancing CO2 fixation by non-photosynthetic autotrophic microorganisms.