装载ACE2的植物来源细胞外囊泡防治PM2.5暴露后细胞损伤
Plant-derived extracellular vesicles loaded with ACE2 prevent cell damage after PM2.5 exposure
叶旋 1钤嫄 2王红云 2周蕾1
作者信息
- 1. 南京医科大学第一附属医院心内科,南京 210029
- 2. 上海大学生命科学学院,上海 200444
- 折叠
摘要
探究装载血管紧张素转化酶2(angiotensin converting enzyme 2,ACE2)的植物来源细胞外囊泡(plant-derived extracellular vesicles,PDEVs)在保护机体抵抗空气污染颗粒物(particulate matter,PM2.5)引发的呼吸系统损伤过程中的作用.基于葡萄提取PDEVs并装载ACE2过表达的质粒,通过CCK8实验、二氢乙锭(dihydroethidium,DHE)染色、蛋白免疫印迹、实时荧光定量多聚酶链反应(polymerase chain reaction,PCR)等方法,探究改造后的PDEVs对PM2.5暴露后人支气管上皮细胞(Beas-2B)的保护效果.PM2.5暴露会造成Beas-2B细胞氧化应激、细胞凋亡增加,以及炎症因子增加,而装载ACE2的PDEVs能够有效递送ACE2,从而缓解PM2.5诱导的细胞损伤.因此,PDEVs可作为潜在的递送载体,防治大气PM2.5暴露引发的细胞凋亡及损伤.
Abstract
To investigate the efficacy of plant-derived extracellular vesicles(PDEVs)loaded with angiotensin-converting enzyme 2(ACE2)in mitigating respiratory system dam-age induced by particulate matter(PM2.5),we extracted PDEVs from grapes and loaded them with ACE2 overexpression plasmids.The effect of the modified PDEVs on human bronchial epithelial cells(Beas-2B)after exposure to PM2.5 was investigated using CCK8 assays,dihydroethidium(DHE)staining,western blotting,and real-time quantitative poly-merase chain reaction(PCR).Exposure to PM2.5 increases oxidative stress,apoptosis,and inflammatory factor production in Beas-2B cells.PDEVs loaded with ACE2 effectively delivered ACE2 to cells and thereby alleviated PM2.5-induced cell damage including apop-tosis,oxidative stress,and inflammation.PDEVs thus show potential for use as carriers of genes that can prevent apoptosis and damage caused by atmospheric PM2.5 exposure.
关键词
细胞外囊泡/血管紧张素转化酶2/PM2.5/葡萄Key words
extracellular vesicles/angiotensin-converting enzyme 2(ACE2)/PM2.5/grapes引用本文复制引用
基金项目
国家自然科学基金面上项目(81970723)
上海市晨光计划(20CG46)
出版年
2024
NETL
NSTL
万方数据