The effect of exercise on platelet-activating factor metabolism in the livers of rats fed high-fat diet
This paper aimed to investigate the effects of exercise on hepatic platelet-activating factor(PAF)metabolism in rats fed a high-fat diet.Thirty-two male Sprague-Dawley(SD)rats were divided into control group(C),high-fat diet group(H),exercise group(EC),and high-fat diet+exercise group(EH).Serum lipids,glucose,insulin and markers of hepatic injury after a 16-week dietary and/or exercise intervention(60 min/day,6 times/week)were measured by biochemical analysis;liver lipidomic profiles were analyzed by liquid chromatograph-mass spectrometer(LC-MS).Gene and protein expression of enzymes related to PAF metabolism were deter-mined by qPCR and Western blot respectively.The results showed that high-fat diet feeding significantly increased the levels of low-density lipoprotein-cholesterol(LDL-C)and liver injury markers including purine nucleoside phosphorylase(PNP)and malondi-aldehyde(MDA)in rats,which were decreased by exercise.Furthermore,high-fat diet feeding significantly increased the hepatic PAF content,which was also attenuated by exercise.In addition,although high-fat diet treatment resulted in an increase in the expression of both PAF synthetase(PAF-CPT and PLA2)and hydrolase(Lp-PLA2 and PAF-AH(Ⅱ)),induction of PAF synthetase was much greater than that of PAF hydrolase.While exercise increased the expression of Lp-PLA2 and PAF-AH(Ⅱ)and decreased the expression of PAF-CPT and PLA2,key PAF synthesizing enzymes.In conclusion,high-fat diet-induced increase in hepatic PAF content is mainly due to the increase of its pathological synthesis at the translational level.Exercise reduces hepatic PAF content in high-fat fed rats by increasing PAF hydrolysis and decreasing its synthesis.
万方数据
维普
