Preparation and characterization of collagen and its nanofibers
Using sheepskin as raw material,sheepskin collagen is extracted by acid method,and then sheepskin collagen/polyvinyl alcohol nanofiber membrane is prepared by electrospinning technology.The effects of hydrochloric acid concentration,hydrolysis time and liquid-to-material ratio on collagen extraction rate are discussed by single-factor experiments and response surface optimization experiments.The extracted sheepskin collagen is characterized by UV,FT-IR and SEM tests,and then the electrospun collagen/polyvinyl alcohol nanofiber membrane is characterized by FT-IR,thermal stability,in vitro degradation and mechanical property tests.The results show that the optimum process conditions for acid extraction of sheepskin collagen are hydrochloric acid concentration of 12％,hydrolysis time of 36 h and liquid-material ratio of 11 mL/g,and the actual extraction rate is 74.56％.The extracted sheepskin collagen structure is relatively complete and has the characteristics of collagen.The composite nanofiber membrane has both the chemical structure of collagen and the chemical structure of polyvinyl alcohol,which belongs to physical composites.The addition of collagen is beneficial to improve the thermal stability of the composite film,accelerate the degradation of the composite film,and also increase the breaking strength and elastic modulus of the composite film.
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