胶原蛋白及其纳米纤维的制备与性能表征
Preparation and characterization of collagen and its nanofibers
姚丹丹 1宋秘钊 2韩洪帅 1李家鑫 1王彦珍1
作者信息
- 1. 齐齐哈尔大学轻工与纺织学院,黑龙江齐齐哈尔 161006
- 2. 齐齐哈尔大学轻工与纺织学院,黑龙江齐齐哈尔 161006;齐齐哈尔大学寒区麻及制品教育部工程研究中心,黑龙江齐齐哈尔 161006
- 折叠
摘要
以羊皮为原料,采用酸法提取羊皮胶原蛋白,然后用静电纺丝技术制备羊皮胶原蛋白/聚乙烯醇纳米纤维膜.利用单因素试验和响应面优化试验讨论了盐酸质量分数、水解时间、液料比对胶原蛋白提取率的影响,通过UV、FTI-IR和SEM测试对提取的羊皮胶原蛋白进行表征,再通过FT-IR、热稳定性、体外降解和机械性能测试对静电纺胶原蛋白/聚乙烯醇纳米纤维膜进行表征.结果表明:酸法提取羊皮胶原蛋白的最佳工艺条件为盐酸质量分数12％、水解时间36 h、液料比11 mL/g,得到实际提取率为74.56％.提取的羊皮胶原蛋白结构较完整,具有胶原蛋白的特性.复合纳米纤维膜既具备胶原蛋白的化学结构,又具有聚乙烯醇的化学结构,属于物理复合.添加胶原蛋白有利于提高复合膜的热稳定性,加速复合膜的降解,也增加了复合膜的断裂强力和弹性模量.
Abstract
Using sheepskin as raw material,sheepskin collagen is extracted by acid method,and then sheepskin collagen/polyvinyl alcohol nanofiber membrane is prepared by electrospinning technology.The effects of hydrochloric acid concentration,hydrolysis time and liquid-to-material ratio on collagen extraction rate are discussed by single-factor experiments and response surface optimization experiments.The extracted sheepskin collagen is characterized by UV,FT-IR and SEM tests,and then the electrospun collagen/polyvinyl alcohol nanofiber membrane is characterized by FT-IR,thermal stability,in vitro degradation and mechanical property tests.The results show that the optimum process conditions for acid extraction of sheepskin collagen are hydrochloric acid concentration of 12％,hydrolysis time of 36 h and liquid-material ratio of 11 mL/g,and the actual extraction rate is 74.56％.The extracted sheepskin collagen structure is relatively complete and has the characteristics of collagen.The composite nanofiber membrane has both the chemical structure of collagen and the chemical structure of polyvinyl alcohol,which belongs to physical composites.The addition of collagen is beneficial to improve the thermal stability of the composite film,accelerate the degradation of the composite film,and also increase the breaking strength and elastic modulus of the composite film.
关键词
羊皮/胶原蛋白/聚乙烯醇/静电纺丝/纳米纤维/提取Key words
sheepskin/collagen/polyvinyl alcohol/electrospinning/nanofiber/extraction rate引用本文复制引用
基金项目
高教强省产业化引导资金项目(150112222011)
省教育厅基本科研业务费项目(130212222125)
出版年
2024
NETL
NSTL
万方数据