The sandwich assay was used to construct a test strip based on the colloidal gold labeled aptamer.The purpose was to develop a practical As(Ⅲ)detection application device.Through the specific combination of As(Ⅲ)and the gold labeled aptamer,As(Ⅲ)would be intercepted at the test line of the test strip.In this study,nano-gold was used as color developing material,and its particle diameter was(13±1)nm.After optimizing the reaction conditions,Satorius CN 140 was selected as the nitrocellulose membrane.The optimal ratio of capture probe streptoavidin and biotin on the control line was 1∶4,and the corresponding concentration of scribing was 10 μmol/L.The optimal ratio of NHS and EDC on the test line was 1∶3,and the optimal concentration of gold standard aptamer was 0.5 μmol/L.The color developing result was observed under the optimal reaction conditions,and the color response signal formed a good gradient relationship with the concentration of As(Ⅲ).The detection limit was 0.1 μmol/L.This test strip had high specificity for As(Ⅲ)and good stability.Its detection cost was less than 1 yuan,which could meet the requirements of real-time detection of As(Ⅲ)for the general population in low-income areas.
NETL
NSTL
万方数据
维普
