Plasma IL-10 level after allogeneic lung transplantation and its relation-ship with bacterial infectious death in clinic
The purpose of this study was to investigate the changes in postoperative plasma IL-10 levels in allogeneic lung trans-plant recipients and its relationship with death due to bacterial infection.To this end,plasma samples were collected from healthy volunteers and lung transplantation recipients who either deceased,survived or lived with a good long-term prognosis.For the deceased group,the immune cell distributions in peripheral blood were analyzed by routine blood analysis.The patho-genic infection status was examined by imaging and the type of pathogenic infection was analyzed by in vitro bacteria culture and by high-throughput genomic sequencing of pathogenic microorganisms.In addition,the concentration of multiple cytokines in all plasma samples was monitored by Luminex multifactor detection.The results showed that in the deceased recipients,postoperative white blood cell count(WBC),absolute neutrophil count(NEUT)and percentage(NEUT％),CRP concentra-tion,and neutrophil-lymphocyte ratio(NLR)were all increased,while red blood cell count(RBC)and platelet count(PLT)were decreased.Moreover,some high-density shadows were observed in the postoperative lungs along with multiple bacterial infections in the blood,bronchoalveolar lavage fluid(BALF),and sputum.Dynamic monitoring of cytokines concentration showed that plasma IL-10 concentration and IL-10/IFN-y ratio were all significantly increased(all P＜0.000 1)in the deceased recipients after allogeneic lung transplantation compared to those of survived recipients,recipients with good prognosis and healthy volunteers.Altogether,this work suggests,for the first time,that the change in plasma IL-10 level is highly related with increased bacterial infection and mortality in lung transplantation recipients.
万方数据
维普
