Effect of CD47 on chemokine secretion of ovarian cancer cells
To investigate the effect of CD47 knockdown on the migration,proliferation,and chemokine secretion of ovarian cancer cells,small interfering RNA(siRNA)targeting CD47 was transfected to ovarian cancer cells and the knockdown efficiency was confirmed by Western blotting,qRT-PCR,and flow cytometry.Cell proliferation,migration,and invasion were examined using CCK-8,wound healing assay,and the Transwell assay,respectively.qRT-PCR was used to detect the expres-sions of CD47,C-X-C motif chemokine ligand 1(CXCL1),CXCL8,C-C motif chemokine ligand 20( CCL20 )and IL-6.ELISA was used to determine the release of IL-6 and CXCL8.Transwell was employed to study macrophage invasion of CD47 knockdown ovarian cancer cells.The results showed that the expressions of chemokines CXCL1,CXCL3,CXCL8,CCL20,and IL-6 in ovarian cancer cells were reduced when CD47 was knocked down,along with the reduction of the migration capacity of macrophages towards ovarian cancer cells.CD47 knockdown did not affect the proliferation,migration,or invasion of ovarian cancer cells.Our findings suggest that CD47 in ovarian cancer cells can affect the migration ability of macrophages by regulating their chemokine expression.
万方数据
维普
